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  Indian J Med Microbiol
 

Figure 1: Chromatogram RP-HPLC with photodiode array DETECTOR. (a) Chromatogram SF1 Flavonoid collecting at RT 32.973 with gradient mobile phase of 100% water to 100% Acetonitrile (1 ml/min). (b) Identification of purity of flavonoid (75,58%) at RT 3.281 with isocratic mobile phase of 40% water: 60% Acetonitrile (1 ml/min). (c) Maximum wavelength of flavonoids seen with Photodiode Array Detector is 273.5 and 376 nm

Figure 1: Chromatogram RP-HPLC with photodiode array DETECTOR. (a) Chromatogram SF1 Flavonoid collecting at RT 32.973 with gradient mobile phase of 100% water to 100% Acetonitrile (1 ml/min). (b) Identification of purity of flavonoid (75,58%) at RT 3.281 with isocratic mobile phase of 40% water: 60% Acetonitrile (1 ml/min). (c) Maximum wavelength of flavonoids seen with Photodiode Array Detector is 273.5 and 376 nm