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  Indian J Med Microbiol
 

Figure 4: (a) (i) HER-2 expression of test compounds at various concentrations to MCF-7/HER-2 cells. Cells without treatment (control) and cells with treatment (N-benzoyl-3-allylthiourea and allylthiourea) with concentrations of 100, 250, 500, 750, and 1000 uM for 24 h, analyzed by western blot method. (ii) Analysis of the results was carried out using ImageJ® software to measure the intensity of the HER-2 expression band on the MCF-7/HER-2 cell line compared to the control. (b) Expression and localization of p65 (nuclear factor kappa B) due to the treatment of N-benzoyl-3-allylthiourea samples on MCF-7/HER-2 cells for 24 h by western blot method at N-benzoyl-3-allylthiourea concentrations of 750 uM and 1000 uM

Figure 4: (a) (i) HER-2 expression of test compounds at various concentrations to MCF-7/HER-2 cells. Cells without treatment (control) and cells with treatment (N-benzoyl-3-allylthiourea and allylthiourea) with concentrations of 100, 250, 500, 750, and 1000 uM for 24 h, analyzed by western blot method. (ii) Analysis of the results was carried out using ImageJ® software to measure the intensity of the HER-2 expression band on the MCF-7/HER-2 cell line compared to the control. (b) Expression and localization of p65 (nuclear factor kappa B) due to the treatment of N-benzoyl-3-allylthiourea samples on MCF-7/HER-2 cells for 24 h by western blot method at N-benzoyl-3-allylthiourea concentrations of 750 uM and 1000 uM