Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 13  |  Issue : 3  |  Page : 161--165

Production, separation, and antimicrobial activity assessment of pristinamycin produced using date fruit extract as substrate


Siddig H Hamad1, Salah M Aleid2, Rafe E Elgorashe3, Mohammed Y Babker1 
1 Department of Food and Nutrition Sciences, College of Agric and Food Sciences, Hofuf, Saudi Arabia
2 Department of Food and Nutrition Sciences, College of Agric and Food Sciences; Date Palm Research Center of Excellence, King Faisal University, Hofuf, Saudi Arabia
3 Department of Chemistry, College of Science, King Faisal University, Hofuf, Saudi Arabia

Correspondence Address:
Prof. Salah M Aleid
Department of Food and Nutrition Sciences, College of Agric and Food Sciences, Hofuf; Date Palm Research Center of Excellence, King Faisal University, Hofuf
Saudi Arabia

Date fruits extract was assessed as substrate for pristinamycin production in shake flasks using Streptomyces pristinaespiralis DSMZ 40338 as production organism. A process of microfiltration, concentration, and solvent extraction was used to recover the antibiotic from the fermentation broth and its antimicrobial activity was tested using a well assay method. During a fermentation period of 120 h, the bacterium consumed approximately 28.4 g/l sugars, grew from 0.4 g/l to 4.0 g/l dry weight, and produced 51.0 mg/L pristinamycin. The microfiltration, concentration, and ethyl acetate solvent extraction applied resulted in 71% pristinamycin recovery. The antibiotic showed inhibition capacity comparable to that of the pristinamycins IA and IIA standards. The inhibited bacteria were Staphylococcus aureus, Escherichia coli, and Enterococcus faecium. Clear inhibition zones of 13.3–19.6 mm diameter were formed.


How to cite this article:
Hamad SH, Aleid SM, Elgorashe RE, Babker MY. Production, separation, and antimicrobial activity assessment of pristinamycin produced using date fruit extract as substrate.J Adv Pharm Technol Res 2022;13:161-165


How to cite this URL:
Hamad SH, Aleid SM, Elgorashe RE, Babker MY. Production, separation, and antimicrobial activity assessment of pristinamycin produced using date fruit extract as substrate. J Adv Pharm Technol Res [serial online] 2022 [cited 2022 Aug 14 ];13:161-165
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2022;volume=13;issue=3;spage=161;epage=165;aulast=Hamad;type=0