Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 12  |  Issue : 3  |  Page : 222--226

Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F pruski leaves


Vivi Mardina1, Syafruddin Ilyas2, Halimatussakdiah Halimatussakdiah3, Tisna Harmawan3, Masitta Tanjung2, Faridah Yusof4 
1 Department of Biology, Faculty of Engineering, Universitas Samudra, 24416 Kota Langsa, Aceh, Indonesia
2 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, 20155, Medan, Indonesia
3 Department of Chemistry, Faculty of Engineering, Universitas Samudra, 24416 Kota Langsa, Aceh, Indonesia
4 Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia

Correspondence Address:
Mrs. Vivi Mardina
Department of Biology, Faculty of Engineering, Universitas Samudra, 24416 Langsa, Aceh
Indonesia

This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial – using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity – using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 μg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 μg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.


How to cite this article:
Mardina V, Ilyas S, Halimatussakdiah H, Harmawan T, Tanjung M, Yusof F. Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F pruski leaves.J Adv Pharm Technol Res 2021;12:222-226


How to cite this URL:
Mardina V, Ilyas S, Halimatussakdiah H, Harmawan T, Tanjung M, Yusof F. Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F pruski leaves. J Adv Pharm Technol Res [serial online] 2021 [cited 2021 Dec 3 ];12:222-226
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2021;volume=12;issue=3;spage=222;epage=226;aulast=Mardina;type=0