Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 4  |  Page : 226--232

Accelerated wound healing ability of Jatropha sap by iota carrageenan-poly (vinyl alcohol) hydrogel film


Nurain Thomas1, Agus Rusdin2, Madania Tulsyahra1, Nasrul Wathoni4, Asep Kuswandi3 
1 Department of Pharmacy, Faculty of Sports and Health, Universitas Negeri Gorontalo, Gorontalo, Indonesia
2 Department of Pharmacy, Faculty of Sports and Health, Universitas Negeri Gorontalo, Gorontalo; Department of Pharmaceutical and Technology Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia
3 Tasikmalaya Health Polytechnic of Ministry of Health, Tasikmalaya, Indonesia

Correspondence Address:
Dr. Agus Rusdin
Department of Pharmacy, Faculty of Sports and Health, Universitas Negeri Gorontalo, Gorontalo 96128; Department of Pharmaceutical and Technology Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang 45363
Indonesia

Jatropha sap (JTS), an important fluid carried in xylem and phloem tubes of Jatropha multifida L. plant, has good wound healing property. However, physicochemical stability of JTS needs to be improved in order for it to be useful as a topical wound-healing agent. In this study, we developed an iota carrageenan-polyvinyl alcohol (IC-PVA) hydrogel film (HF) as a carrier of JTS and evaluated its wound-healing ability. The characterization of JTS secondary metabolites by ultraviolet-Vis spectrophotometry suggested presence of flavonoid, saponin, and alkaloids in the sap. We successfully extracted IC from Euchima spinosum using alkaline solvent at 80°C–90°C with calcium chloride as the precipitator. The result of computer simulation using Discovery Studio software and Autodock Tools showed the presence of hydrogen bonding interaction of IC-PVA. IC-PVA/JTS HF with excellent physical properties including high swelling ratio (246.32%) and high gel fraction (16.75%). In addition, irritation test in mice confirmed the absence of hypersensitivity reaction, redness, and allergic reactions. Interestingly, IC-PVA/JTS HF significantly accelerated wound healing when compared to the nontreated group/control with 98% wound closure by 10 days. These results suggest that IC-PVA HF has improves wound-healing ability of JTS.


How to cite this article:
Thomas N, Rusdin A, Tulsyahra M, Wathoni N, Kuswandi A. Accelerated wound healing ability of Jatropha sap by iota carrageenan-poly (vinyl alcohol) hydrogel film.J Adv Pharm Technol Res 2020;11:226-232


How to cite this URL:
Thomas N, Rusdin A, Tulsyahra M, Wathoni N, Kuswandi A. Accelerated wound healing ability of Jatropha sap by iota carrageenan-poly (vinyl alcohol) hydrogel film. J Adv Pharm Technol Res [serial online] 2020 [cited 2020 Nov 28 ];11:226-232
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=4;spage=226;epage=232;aulast=Thomas;type=0