Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 10  |  Issue : 4  |  Page : 149--154

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells


Amaq Fadholly1, Annise Proboningrat1, Regina Purnama Dewi Iskandar2, Fedik Abdul Rantam3, Sri Agus Sudjarwo4 
1 Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
2 Doctoral Student of Medical Science, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
3 Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
4 Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

Correspondence Address:
Prof. Sri Agus Sudjarwo
Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya
Indonesia

This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.


How to cite this article:
Fadholly A, Proboningrat A, Dewi Iskandar RP, Rantam FA, Sudjarwo SA. In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells.J Adv Pharm Technol Res 2019;10:149-154


How to cite this URL:
Fadholly A, Proboningrat A, Dewi Iskandar RP, Rantam FA, Sudjarwo SA. In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells. J Adv Pharm Technol Res [serial online] 2019 [cited 2021 Dec 6 ];10:149-154
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=4;spage=149;epage=154;aulast=Fadholly;type=0