Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 8  |  Issue : 2  |  Page : 52--58

Simultaneous micronization and purification of bioactive fraction by supercritical antisolvent technology


Stevanus Hiendrawan, Bambang Veriansyah, Edward Widjojokusumo, Raymond R Tjandrawinata 
 Section of Advanced Technology Development, Research Innovation and Invention, Dexa Laboratories of Biomolecular Sciences, Cikarang, Indonesia

Correspondence Address:
Raymond R Tjandrawinata
Dexa Laboratories of Biomolecular Sciences, Industri Selatan V, Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java 17750
Indonesia

Simultaneous micronization and purification of DLBS3233 bioactive fraction, a combination of two Indonesian herbals Lagerstroemia speciosa and Cinnamomum burmannii has been successfully performed via supercritical anti-solvent (SAS) technology. The objective of the present study was to investigate the effectiveness of SAS technology to micronize and reduce coumarin content of DLBS3233. The effects of four SAS process parameters, i.e. pressure, temperature, concentration and solution flow rate on particle formation were investigated. In SAS process, DLBS3233 was dissolved in dimethylformamide (DMF) as the liquid solvent. The solution was then pumped through a nozzle into a chamber simultaneously with supercritical carbon dioxide (SC-CO2) which acts as the anti-solvent, resulting in DLBS3233 precipitation. Physicochemical properties of unprocessed DLBS3233 and SAS-processed DLBS3233 particles were analyzed using scanning electron microscopy (SEM) and high pressure liquid chromatography (HPLC). Total polyphenol content (TPC) was also analyzed.Particles with mean particle size ranging from 0.107±0.028 μm to 0.298±0.138 μm were obtained by varying the process parameters. SAS-processed DLBS3233 particles showed no coumarin content in all experiments studied in this work. Results of TPC analysis revealed no significant change in SAS-processed DLBS3233 particles compared to unprocessed DLBS3233. Nano-sized DLBS3233 particles with no coumarin content have been successfully produced using SAS process. This study demonstrates the ability of SAS for processing herbal medicine in single step process.


How to cite this article:
Hiendrawan S, Veriansyah B, Widjojokusumo E, Tjandrawinata RR. Simultaneous micronization and purification of bioactive fraction by supercritical antisolvent technology.J Adv Pharm Technol Res 2017;8:52-58


How to cite this URL:
Hiendrawan S, Veriansyah B, Widjojokusumo E, Tjandrawinata RR. Simultaneous micronization and purification of bioactive fraction by supercritical antisolvent technology. J Adv Pharm Technol Res [serial online] 2017 [cited 2021 Sep 16 ];8:52-58
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2017;volume=8;issue=2;spage=52;epage=58;aulast=Hiendrawan;type=0