Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 7  |  Issue : 3  |  Page : 75--79

Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants


Eskandar Moghimipour1, Sayyed Abolghassem Sajadi Tabassi2, Mohammad Ramezani3, Somayeh Handali1, Raimar Lobenberg4 
1 Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4 Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada

Correspondence Address:
Somayeh Handali
Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz
Iran

The aim of this study was to investigate the influence of absorption enhancers in the uptake of hydrophilic compounds. The permeation of the two hydrophilic drug models gentamicin and 5 (6)-carboxyfluorescein (CF) across the brush border membrane vesicles and Caco-2 cell lines were evaluated using total saponins of Acanthophyllum squarrosum, Quillaja saponaria, sodium lauryl sulfate, sodium glycocholate, sodium taurodeoxycholate , and Tween 20 as absorption enhancers. Transepithelial electrical resistance (TEER) measurement was utilized to assess the paracellular permeability of cell lines. Confocal laser scanning microscopy (CLSM) was performed to obtain images of the distribution of CF in Caco-2 cells. These compounds were able to loosen tight junctions, thus increasing paracellular permeability. CLSM confirmed the effect of these absorption enhancers on CF transport across Caco-2 lines and increased the Caco-2 permeability via transcellular route. It was also confirmed that the decrease in TEER was transient and reversible after removal of permeation enhancers.


How to cite this article:
Moghimipour E, Tabassi SA, Ramezani M, Handali S, Lobenberg R. Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants.J Adv Pharm Technol Res 2016;7:75-79


How to cite this URL:
Moghimipour E, Tabassi SA, Ramezani M, Handali S, Lobenberg R. Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants. J Adv Pharm Technol Res [serial online] 2016 [cited 2021 Dec 6 ];7:75-79
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2016;volume=7;issue=3;spage=75;epage=79;aulast=Moghimipour;type=0