Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 6  |  Issue : 3  |  Page : 108--113

A reverse phased high-pressure liquid chromatographic method for the estimation of a poisonous matter in Strychnos nux-vomica


Achu Hashim1, Rafique Mohammed2, Dilshad Umar2, Vaswani R Veena1, Basheer Bahija2, Baroudi Kusai2 
1 Department of Forensic Medicine and Toxicology, Yenepoya Medical College, Mangalore, India
2 Department of Cleft and Craniofacial Surgery, Yenepoya University, Mangalore, India

Correspondence Address:
Dilshad Umar
Al Farabi Colleges, Riyadh, Saudi Arabia

Seeds of Strychnos nux-vomica were subjected to preliminary phytochemical tests and its presence was confirmed by thin layer chromatography (TLC) method. The TLC profile of the methanolic extract of seeds of S. nux-vomica was developed using the solvent system toluene:chloroform:methanol in the ratio 8:2:1. The plate was observed in visible light after spraying with Dragendorff«SQ»s reagent (specific method). High-performance liquid chromatography (HPLC) profile of the methanolic extracts of S. nux-vomica was developed, and the amount of strychnine seems to be 0.36% (w/w) in the seeds. The TLC and HPLC profiles developed are very valuable for the identification of the original drug from their adulterants. The TLC profile identifies the presence of strychnine in the plant material. The quantification method for the strychnine in the seeds can be used for the quality standardization of the raw drug because the strychnine is reported to have some toxicity.


How to cite this article:
Hashim A, Mohammed R, Umar D, Veena VR, Bahija B, Kusai B. A reverse phased high-pressure liquid chromatographic method for the estimation of a poisonous matter in Strychnos nux-vomica.J Adv Pharm Technol Res 2015;6:108-113


How to cite this URL:
Hashim A, Mohammed R, Umar D, Veena VR, Bahija B, Kusai B. A reverse phased high-pressure liquid chromatographic method for the estimation of a poisonous matter in Strychnos nux-vomica. J Adv Pharm Technol Res [serial online] 2015 [cited 2021 Dec 7 ];6:108-113
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2015;volume=6;issue=3;spage=108;epage=113;aulast=Hashim;type=0