Journal of Advanced Pharmaceutical Technology & Research

SHORT COMMUNICATION
Year
: 2014  |  Volume : 5  |  Issue : 3  |  Page : 147--149

A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit


Akhilesh Mishra1, Gayatri Dewangan2, W Ramdas Singh3, Sarbani Hazra4, Tapan Kumar Mandal1 
1 Department of Veterinary Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, Mhow, Madhya Pradesh, India
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, Agartala, Tripura, India
4 Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India

Correspondence Address:
Tapan Kumar Mandal
Department of Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, Kolkata - 700 037, West Bengal
India

This article describes a simple and rapid method for determination of curcumin (diferuloylmethane) in aqueous humor of rabbit using high-performance liquid chromatography (HPLC). Analysis was performed using a C-18 column (250 × 4.6 mm, 5 μ luna) by isocratic elution with a mobile phase containing 25 mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40:60) and detection at 424 nm using a photodiode array (PDA) detector for curcumin. The regression data for curcumin showed a good linear relationship with r 2 > 0.998 over the concentration range of 0.1-10 μg ml−1 . Relative standard deviations (RSD) for the intraday and interday coefficient of variations for the assay were less than 5.0 and 8.5, respectively. The recovery of the method was between 79.8-83.6%. The quantification limit of the method for curcumin was 0.01 μg ml−1 . This method has good accuracy, precision, and quantitation limit. It is also concluded that the method is useful for measuring very low curcumin concentrations in aqueous humor.


How to cite this article:
Mishra A, Dewangan G, Singh W R, Hazra S, Mandal TK. A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit.J Adv Pharm Technol Res 2014;5:147-149


How to cite this URL:
Mishra A, Dewangan G, Singh W R, Hazra S, Mandal TK. A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit. J Adv Pharm Technol Res [serial online] 2014 [cited 2021 Nov 28 ];5:147-149
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2014;volume=5;issue=3;spage=147;epage=149;aulast=Mishra;type=0