Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 4  |  Issue : 3  |  Page : 160--165

Method development for Lawsone estimation in Trichup herbal hair powder by high-performance thin layer chromatography


Maunang M Patel1, Bhavna R Solanki2, Nilesh C Gurav2, Prateek H Patel2, Shweta S Verma1 
1 Department of Pharmacy, Sumandeep Vidyapeeth, Pipariya, Waghodiya, Gujarat, India
2 Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.), 896/A, G.I.D.C, Makarpura, Vadodara, Gujarat, India

Correspondence Address:
Bhavna R Solanki
Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.) 896 /A, G.I.D.C., Makarpura, Vadodara - 390 010, Gujarat
India

A simple, specific, accurate, precise and robust high-performance thin-layer chromatographic method has been developed and validated for estimation of Lawsone in Trichup herbal hair powder (coded as a THHP), polyherbal formulation. The chromatographic development was carried out on aluminum plates pre-coated with silica gel 60F 254 and good resolution was achieved with Toluene: Ethyl acetate: Glacial acetic acid (8:1:1 v/v/v) as mobile phase. Lawsone detection was carried out densitometrically at 277 nm and obtained retardation factor value was 0.46 ± 0.02. The method was validated with respect to specificity, linearity, accuracy, precision and robustness. The calibration curve was achieved to be linear over a range of 5-60 μg/ml and regression coefficient was obtained 0.998. Accuracy of chromatographic method was evaluated by standard addition method; recovery was obtained 99.25 ± 0.61% . The peak purity of Lawsone was achieved 0.999 r. Relative standard deviation for intraday and inter-day precision was 0.37-0.56% and 0.42-0.55%, respectively. The limit of detection and limit of quantification of the Lawsone were found to be 1.08 μg/m land 3.28 μg/ml, respectively. This result shows that the method was well validated. In the present study, the Lawsone content was found 0.322 ± 0.014% in THHP. This study reveals that the proposed high performance thin layer chromatography method is accurate, fast and cost- effective for routine estimation of Lawsone in polyherbal formulation.


How to cite this article:
Patel MM, Solanki BR, Gurav NC, Patel PH, Verma SS. Method development for Lawsone estimation in Trichup herbal hair powder by high-performance thin layer chromatography.J Adv Pharm Technol Res 2013;4:160-165


How to cite this URL:
Patel MM, Solanki BR, Gurav NC, Patel PH, Verma SS. Method development for Lawsone estimation in Trichup herbal hair powder by high-performance thin layer chromatography. J Adv Pharm Technol Res [serial online] 2013 [cited 2021 Dec 7 ];4:160-165
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2013;volume=4;issue=3;spage=160;epage=165;aulast=Patel;type=0