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Year : 2023  |  Volume : 14  |  Issue : 1  |  Page : 51-55

Bioassay for total serum bioactivity of Atractylodes lancea

Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand

Correspondence Address:
Prof. Kesara Na-Bangchang
Chulabhorn International College of Medicine, Thammasat University, 99 Moo 18, Paholyothin Road, Klong Luang, Pathumtanee
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/japtr.japtr_431_22

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The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/ml) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.

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