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ORIGINAL ARTICLE
Year : 2023  |  Volume : 14  |  Issue : 1  |  Page : 34-38  

Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city


Department of Biology, College of Education for Pure Science, University of Kirkuk, Kirkuk, Iraq

Date of Submission02-Aug-2022
Date of Decision06-Sep-2022
Date of Acceptance21-Sep-2022
Date of Web Publication20-Jan-2023

Correspondence Address:
Dr. Najdat Bahjat Mahdi
Department of Biology, College of Education for Pure Science, University of Kirkuk, Kirkuk
Iraq
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/japtr.japtr_508_22

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  Abstract 


Staphylococcus aureus resides naturally in the nasal cavity of healthy individuals, including those working in restaurants, so they may be a source for spreading this bacterium to restaurant customers directly or indirectly through cooked meals. This bacterium has several virulence factors enabling it to cause many diseases in different parts of the body. It has also the capability to resist conventional antibiotics including methicillin. To investigate methicillin-resistant S. aureus (MRSA), 170 nasal swabs were collected from food preparation workers in 30 restaurants (5–6 workers in each restaurant) in Kirkuk city. After collection, the samples were directly transferred to the laboratory and cultured on selective media like mannitol salt agar (MSA). Microbiological examination including morphological, biochemical, and confirmatory tests showed that 24/170 of collected samples were positive for S. aureus with a rate of 14.12%. Among 24 isolates, 20 (83.3%) belonged to MRSA. All isolates were resistant to oxacillin and penicillin (100%), whereas sensitive to other antibiotics (gentamicin, chloramphenicol, and rifampicin). Polymerase chain reaction exhibited that 13 (65%) of MRSA isolates have toxic shock syndrome toxin-1 gene and only 4 (20%) have Panton–Valentine leukocidin gene.

Keywords: Kirkuk city, methicillin-resistant Staphylococcus aureus, nasal carriage, Panton–Valentine leukocidin, restaurant workers, toxic shock syndrome toxin-1


How to cite this article:
Mahdi NB. Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city. J Adv Pharm Technol Res 2023;14:34-8

How to cite this URL:
Mahdi NB. Prevalence of panton–Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city. J Adv Pharm Technol Res [serial online] 2023 [cited 2023 Feb 4];14:34-8. Available from: https://www.japtr.org/text.asp?2023/14/1/34/368246




  Introduction Top


Staphylococcus aureus is considered one of the main problematic pathogens that human beings is facing. Each person in one way or another would have S. aureus infection during a lifetime. The severity of the infection varies from food poisoning to some dermal infections and in some cases would cause mortality.[1] The nose is the natural site of colonization of S. aureus, and near of 30% of people are colonized at any one time. Therefore, those people develop the risk of skin infections caused by S. aureus.[2] Bacterial pathogenicity is associated with the secretion of several virulence factors such as clumping factors, protein A, toxic shock syndrome (TSS), and Panton–Valentine leukocidin (PVL).[3] Among these exotoxins, the function of PVL in the pathogenesis is exactly unknown until it was remarked that strains that retain PVL genes were considerably associated with, sepsis, necrotizing pneumonia, and skin and soft-tissue infections. In addition, PVL consists of two components, encoded by prophage, which attack individual monocytes, phagocytic cells, and host polymorphonuclear leukocytes then lead to tissue damage. Furthermore, the existence of PVL genes was recognized as a conceivable marker of community-associated methicillin-resistant S. aureus (MRSA) isolates, whereas TSST-1 is correlated with acute multisystem diseases.[4] This bacteria can also be transmitted from food makers to food items through unhygienic practices such as coughing or sneezing or dealing with food before hand cleaning.[5] Food makers can act as a carrier of virulent isolates and maybe a way of their transport to food. Newly years predominance of antibiotic-resistant strains are given widespread attention, especially MRSA strains because they are not sensitive to all obtainable penicillins and beta-lactams and important reason for morbidity and mortality worldwide.[6]

Almost all previous studies in our city related to food poisoning have paid attention to screening isolates of S. aureus for enterotoxins in food industry workers and the resistance or sensitivity of these microorganisms to various antibiotics. Due to the deficiency of information about the presence of PVL and TSST-1 genes in methicillin-resistant isolates in these bacteria, this study aimed to the determined of antibiotic-resistant pattern and the frequency of TSST-1 and PVL genes in the nasal carriage of MRSA of restaurant workers in Kirkuk City, Northern Iraq.


  Materials and Methods Top


Sample collection

One hundred and seventy nasal swabs were randomly collected from workers in the kitchens of 30 restaurants, 5–6 workers in each restaurant and it was transferred directly to the Microbiology Laboratory at one educational institution for the period from December 1, 2020, to March 1, 2021.

Isolation and identification

The collected samples were placed on MSA and incubated for 18–24 h at 37°C, then phenotypic tests were performed on the growing colonies, which included the size, shape, and color of the colonies and gram stain, followed by biochemical diagnostic tests, which included catalase coagulase DNase, oxidase.[7],[8] it was also cultured on the blood agar medium to know the extent of production of hemolysin, and the diagnosis was confirmed by VITEK 2 system. Some virulence factors were detected such as their secretion of the enzyme β-lactamase and the cell wall containing protein A.[9],[10] To confirm isolates as methicillin-resistant, cefoxitin (30 μg) disc diffusion test was performed.

Susceptibility test to antibiotics

A sensitivity test was conducted for MRSA isolates to 13 antibiotics commonly used in Kirkuk city. These are penicillin 10 unit, rifampicin 5 μg, oxacillin 1 μg, clindamycin 2 μg, gentamicin 10 μg, erythromycin 15 μg, tetracycline 30 μg, ciprofloxacin 5 μg, and levofloxacin 5 μg. Chloramphenicol 3 μg, clindomycin 10 μg, trimethoprim. sulfamethoxazole (1.25/23.75) μg, trimethoprim 5 μg, and vancomycin 10 μg, all suppliers were from Oxford UK. The sensitivity and resistance of isolates to the antibiotics done by the disk diffusion method were estimated as reported by Kirby-Bayer.[11] and based on what was mentioned by the clinical laboratory standard institute.[12]

DNA extraction and toxic shock syndrome toxin-1 and Panton–Valentine leukocidin genes detection

Bacterial DNA was extracted from 20 MRSA isolates using a DNA extraction kit supplied by Promega Company. The concentration of extracted DNA was also estimated using a BioDrop Spectrophotometer (Cambridge CB4 England) and the purity of the product was assessed at a wavelength of 260/280 nm. Polymerase chain reaction (PCR) and gel electrophoresis were performed according to the manufacturer's agreement. The primers were used in the present study to a determination TSS-1 and PVL genes according to Khosravi et al. and Koosha et al.[13],[14] as shown in [Table 1]. PCR was performed based on the following: basic denaturation for 5 minutes at 95°C, pursued by 30 cycles for the 30 s at 95°C, and 72°C for 30 s with a finale extension at 72°C for 7 min. PCR results were detected using gel electrophoresis at 100 V/mA for 75 min. The bands were detected by ultraviolet rays and photocopied.
Table 1: Primer sequences and lengths of amplified DNA products

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  Results and Discussion Top


To investigate the MRSA, 170 swabs were taken from the noses of cooks in 30 restaurants, 5–6 workers at each restaurant in Kirkuk, for the period from January 1, 2020, to May 1, 2020. Samples were immediately transferred to the laboratory and cultured on MSA and incubated for 18–24 h at 37°C.

Twenty-four of the cultured media gave positive growth at a rate of 14.11%, they were yellow, meaning they were fermented mannitol. Isolates that fermented with mannitol were diagnosed based on phenotypic and microscopic characteristics and biochemical tests. Microscopic examinations showed that these isolates were spherical and gram-positive. All of them were positive for catalase, coagulase, DNase, and protein A, whereas they were negative for oxidase. These results were confirmed by VITEK 2. Among the 24 isolates, 20 (83.3%) belong to MRSA. Inconsistent to Al-Tamim et al. and Vatansever et al.[15],[16] and it was similar to the results found by Rasheed and Hussein.[17] The reason for the similarities and differences may be due to geographical location, environmental conditions, and some social customs. Culturing on blood agar showed that 9/20 (45%) alpha hemolytic isolates, whereas 12 (60%) were positive for the beta-lactamase test.

Sensitivity tests were conducted for MRSA isolates, by disk diffusion method using 13 antibiotics, which were divided according to the effect of these antibiotics into cell wall inhibitors represented by penicillin and oxacillin. Moreover, the protein synthesis inhibitors represented by clindamycin, tetracycline, erythromycin, and chloramphenicol, whereas the nucleic acid inhibitors were levofloxacin, ciprofloxacin, and rifampicin. The results mentioned in [Figure 1] showed the response of the bacterial isolates to the different antibiotics used in the experiment. The highest resistance was observed against oxacillin and penicillin with percentage of 100%. The results are close to what was found by Ahmadi et al.[18] and similar to the results of Abie et al. and Zhu et al.,[19],[20] whereas inconsistent with Gushiken et al.[21]
Figure 1: Antibiotic-resistant pattern of MRSA isolates. P: Penicillin, OX: Oxacillin, GM: Gentamicin, C: Chloramphenicol, TE: Tetracycline, E: Erythromycin, DA: Clindamycin, Cip: Ciprofloxacin, LEV: Levofloxacin, TMP: Trimethoprim, SXT: Trimethoprim/sulfamethoxazole, Va: Vancomycin, RIF: Rifampicin, MRSA: Methicillin-Resistant Staphylococcus aureus

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All isolates were also sensitive to gentamicin and chloramphenicol at 100%, whereas they were resistant to clindamycin, erythromycin, and tetracycline with percentages of 35%, 45%, and 15%, respectively, our results match the results obtained by Najafi Olya et al.[22] Furthermore, all isolates were sensitive to rifampin, an inhibitor of RNA synthesis by 100%. Moreover, they were resistant by 20% to ciprofloxacin and levofloxacin. As for vancomycin, most of them were sensitive to this antibiotic by 90%, whereas the percentage of sensitivity to inhibitors of basic metabolic substances was trimethoprim sulfamethoxazole and trimethoprim 95% incompatible with Rostamzad and Rostamneia.[23] The reason for the sensitivity of some bacterial isolates to the antibiotics under study is that they were able to reach their targets efficiently and thus affect the target site according to the mechanism of action of each antibiotic.

S. aureus pursues several antibiotic resistance strategies including the acquisition of spontaneous point mutations in the original target that reduce antibiotic binding, acquisition of a gene that encodes an enzyme that inactivates the antibiotic, and acquisition of an antibiotic insensitive target that can bypass the effect of the antibiotic by substituting the function of the antibiotic. The original target, expressing a protein or proteins that transport the drug from the cytoplasm, thereby reducing its cytoplasmic concentration, and acquiring mutations that alter the surface properties of the bacteria that, for example, reduce the interaction of the antibiotic with the bacteria.[24]

The results of the polymerase chain reaction for the MRSA strains as in [Figure 2], showed that through 20 isolated MRSA, 13 (65%) were affirmative only for the TSST-1 gene and 4 (20%) were positive only for PVL gene [Figure 3], This was similar to the results of Goudarzi et al.[25] who described a prevalence of the PVL encoded gene of 21.4% within MRSA isolates in Iran. While our results were contrary to what was mentioned by Udo et al.[26] whom isolated MRSA in Kuwait's capital City restaurants from food handlers. The presence of PVL genes coincided with SST-1 in three isolates (15%), whereas 11 isolates contained only the TSST-1 gene. This result is inconsistent with Sato et al.[27]
Figure 2: PCR agarose gel electrophoresis for the detection of the PVL gene (433bp) in MRSA strains. PVL: Panton–Valentine leukocidin, MRSA: Methicillin-resistant Staphylococcus aureus

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Figure 3: PCR agarose gel electrophoresis for the detection of the SST-1 gene (587bp) in MRSA strains. MRSA: Methicillin-Resistant Staphylococcus aureus, SST-1: Shock syndrome toxin, PCR: Polymerase chain reaction

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The recent alarming development is the emergence of strains of Community acquired MRSA (CA-MRSA) with multiantibiotic resistance and containing several important virulence factors such as PVL and TSST that cause many severe and life-threatening diseases. As the name implies, CA-MRSA strains are not restricted to hospitals and are obtained from contact in various education centers such as schools and universities, restaurants, and other community settings. Carriers of nasal MRSA are more susceptible to infection than noncarriers and serve as important reservoirs of infection so the existence of these strains in the food chain means presents a potential health risk to consumers and deserves further monitoring, and we believe that the nasal MRSA colonization screening is essential in infection protection and control and may provide beneficial information to lead antibacterial therapy also there is a need for appropriate food safety training among food handlers and to improve food quality.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Riedel S, Morse SA, Mietzner TA, Miller S. Jawetz Melnick & Adelberg's Medical Microbiology 28 E. USA: McGraw Hill Professional; 2019.  Back to cited text no. 1
    
2.
Levinson W. Review of Medical Microbiology and Immunology. USA: McGraw-Hill Education; 2014.  Back to cited text no. 2
    
3.
Aung MS, San T, Aye MM, Mya S, Maw WW, Zan KN, et al. Prevalence and genetic characteristics of Staphylococcus aureus and staphylococcus argenteus isolates harboring Panton-Valentine leukocidin, enterotoxins, and TSST-1 genes from food handlers in Myanmar. Toxins (Basel) 2017;9:241.  Back to cited text no. 3
    
4.
AlFouzan W, Al-Haddad A, Udo E, Mathew B, Dhar R. Frequency and clinical association of Panton-Valentine leukocidin-positive Staphylococcus aureus isolates: A study from Kuwait. Med Princ Pract 2013;22:245-9.  Back to cited text no. 4
    
5.
Noor-Azira AM, Mohammad-Faid AR, Shuhaimi M, Syafinaz AN, Hamat RA, Malina O. Staphylococcus aureus in food and nares of food handlers in Kuala Pilah, Malaysia. Pertanika J Trop Agric Sci 2012;35:853-62.  Back to cited text no. 5
    
6.
Castro A, Komora N, Ferreira V, Lira A, Mota M, Silva J, et al. Prevalence of Staphylococcus aureus from nares and hands on health care professionals in a Portuguese Hospital. J Appl Microbiol 2016;121:831-9.  Back to cited text no. 6
    
7.
James G, Emeritus C. Microbiology: A Laboratory Manual. 12th ed. USA: SUNY Rockland Community College, Chad Welsh. Lindenwood University; 2020.  Back to cited text no. 7
    
8.
Mahon CR, Lehman DC, Manuselis G.Textbook of Diagnostic Microbiology-E-Book. USA: Elsevier Health Sciences; 2018.  Back to cited text no. 8
    
9.
Luis M, Pezzlo MT, Bittencourt CE, Peterson EM. Color Atlas of Medical Bacteriology. USA: John Wiley and Sons; 2020.  Back to cited text no. 9
    
10.
Cooper RG, Brown GW, Vesey BV. Iodometric detection of staphylococcal penicillinase in clinical microbiology. Aust J Exp Biol Med Sci 1966;44:715-7.  Back to cited text no. 10
    
11.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.  Back to cited text no. 11
    
12.
Weinstein MP, Lewis JS 2nd. The clinical and laboratory standards institute subcommittee on antimicrobial susceptibility testing: Background, organization, functions, and processes. J Clin Microbiol 2020;58:e01864-19.  Back to cited text no. 12
    
13.
Khosravi AD, Hoveizavi H, Farshadzadeh Z. The prevalence of genes encoding leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in Taleghani Hospital, Ahvaz, Iran. Burns 2012;38:247-51.  Back to cited text no. 13
    
14.
Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, Ghorbani Tajandareh S, Imani Fooladi AA. Distribution of TSST-1 and mecA Genes in Staphylococcus aureus isolated from clinical specimens. Jundishapur J Microbiol 2016;9:e29057.  Back to cited text no. 14
    
15.
Al-Tamimi M, Himsawi N, Abu-Raideh J, Abu Jazar D, Al-Jawaldeh H, Al Haj Mahmoud S, et al. Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students. J Infect Dev Ctries 2018;12:326-35.  Back to cited text no. 15
    
16.
Vatansever L, Sezer Ç, Bilge N. Carriage rate and methicillin resistance of Staphylococcus aureus in food handlers in Kars City, Turkey. Springerplus 2016;5:608.  Back to cited text no. 16
    
17.
Rasheed NA, Hussein NR. Methicillin-resistant Staphylococcus aureus carriage rate and molecular characterization of the staphylococcal cassette chromosome mec among Syrian refugees in Iraq. Int J Infect Dis 2020;91:218-22.  Back to cited text no. 17
    
18.
Ahmadi E, Khojasteh M, Mortazavi SM, Khan-Mohammadi F, Kazemnia A, Beheshtipour J, et al. Prevalence of and risk factors for methicillin-resistant Staphylococcus aureus nasal carriage in the West of Iran: A population-based cross-sectional study. BMC Infect Dis 2019;19:899.  Back to cited text no. 18
    
19.
Abie S, Tiruneh M, Abebe W. Methicillin-resistant Staphylococcus aureus nasal carriage among janitors working in hospital and non-hospital areas: A comparative cross-sectional study. Ann Clin Microbiol Antimicrob 2020;19:47.  Back to cited text no. 19
    
20.
Zhu H, Luo H, Zhong Q, Cao X, Gu S, Peng S, et al. Comparison of molecular characteristics between methicillin-resistant and -susceptible Staphylococcus aureus clinical isolates by whole-genome sequencing. Infect Drug Resist 2022;15:2949-58.  Back to cited text no. 20
    
21.
Gushiken CY, Medeiros LB, Correia BP, Souza JM, Moris DV, Pereira VC, et al. Nasal carriage of resistant Staphylococcus aureus in a medical student community. An Acad Bras Cienc 2016;88:1501-9.  Back to cited text no. 21
    
22.
Najafi Olya Z, Najar-Peerayeh S, Yadegar A, Bakhshi B. Clonal diversity and genomic characterization of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus in Tehran, Iran. BMC Infect Dis 2021;21:372.  Back to cited text no. 22
    
23.
Rostamzad A, Rostamneia N. Prevalence of the Panton-Valentine leukocidin gene in clinical isolates of Staphylococcus aureus isolated from hospitals the ilam province of Iran. Avicenna J Clin Microbiol Infect 2016;3:32691.  Back to cited text no. 23
    
24.
Somerville GA. Staphylococcus: Genetics and physiology. USA: Caister Academic Press; 2016.  Back to cited text no. 24
    
25.
Goudarzi M, Goudarzi H, Sá Figueiredo AM, Udo EE, Fazeli M, Asadzadeh M, et al. Molecular characterization of methicillin resistant Staphylococcus aureus strains isolated from intensive care units in Iran: ST22-SCCmec IV/t790 emerges as the major clone. PLoS One 2016;11:e0155529.  Back to cited text no. 25
    
26.
Udo EE, Al-Mufti S, Albert MJ. The prevalence of antimicrobial resistance and carriage of virulence genes in Staphylococcus aureus isolated from food handlers in Kuwait City restaurants. BMC Res Notes 2009;2:108.  Back to cited text no. 26
    
27.
Yamaguchi T, Nakamura I, Sato T, Ono D, Sato A, Sonoda S, et al. Changes in the genotypic characteristics of community-acquired methicillin-resistant Staphylococcus aureus collected in 244 medical facilities in Japan between 2010 and 2018: A nationwide surveillance. Microbiol Spectr 2022;10:e0227221.  Back to cited text no. 27
    


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