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Year : 2022  |  Volume : 13  |  Issue : 2  |  Page : 133-137

Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract

1 Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
2 Department of Cosmetology, Engineering Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
3 Chemistry Division, Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, Malaysia, Asia, Malaysia
4 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, East Kalimantan, Indonesia

Correspondence Address:
Dr. Neneng Siti Silfi Ambarwati
Jl. Rawamangun Muka, Jakarta Timur - 13220
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/japtr.japtr_16_22

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This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl. An in vivo antioxidant with oxidative stress reduction was carried out for 10 days on 25 male Sprague–Dawley rats (divided into five groups). Every day, each group was given positive control, negative control, 5, 10, and 20 mg/200 gr of body weight (BW) of the extract. The blood plasma was taken for malondialdehyde analysis. A phytochemical identification of PEE revealed more compounds, such as flavonoids, alkaloids, steroids, essential oils, and tannin. PEE exhibits significant in vitro radical scavenging activity, with an IC50 value of 126.695 μg/mL. In the in vivo antioxidant with oxidative stress reduction experiments, 5 mg/200 gr BW was the most effective dose, as evidenced by a considerable drop in malondialdehyde level (0.312 nmol/mL) after and before treatment. In conclusion, PPE has the potential to be developed as a herbal antioxidant based on in vitro and in vivo test results.

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