New robustaflavone from Garcinia latissima Miq. leave and Its antibacterial activity

Neneng Siti Silfi Ambarwati; Berna Elya; Amarila Malik; Hanita Omar; Muhammad Hanafi; Islamudin Ahmad

doi:10.4103/japtr.japtr_132_21

Users Online: 1897

ORIGINAL ARTICLE

Year : 2022 | Volume : 13 | Issue : 1 | Page : 50-55

New robustaflavone from Garcinia latissima Miq. leave and Its antibacterial activity

Neneng Siti Silfi Ambarwati¹, Berna Elya², Amarila Malik³, Hanita Omar⁴, Muhammad Hanafi⁵, Islamudin Ahmad⁶
¹ Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Indonesia
² Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
³ Microbiologi-Biotechnology, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
⁴ Centre of Foundation Studies in Science, Chemistry Division, University of Malaya, Kuala Lumpur, Malaysia
⁵ Research Centre for Chemistry, Indonesian Institute of Sciences, South Tangerang, Banten; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Faculty of Pharmacy, Pancasila University, South Jakarta, Jakarta, Indonesia
⁶ Department of Pharmaceutical Sciences, and Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, East Kalimantan, Indonesia

Correspondence Address:
Dr. Islamudin Ahmad
Building of Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, 2^nd Floor, Jalan Kesehatan, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, 75119 East Kalimantan
Indonesia

Source of Support: None, Conflict of Interest: None

Check

DOI: 10.4103/japtr.japtr_132_21

Isolation and determination of antibacterial compounds from plants are essential to obtain a new antibacterial as a substitute for conventional resistant antibiotics. This study aims to isolate and identify a new robustaflavone as antibacterial activity from Garcinia latissima Miq. leave. In this study, the isolation process was carried out using column chromatography followed by preparative thin layer chromatography (TLC) based on the TLC profile. The fraction D was tested for anti-bacterial Bacillus subtilis using the TLC bioautography method. The isolates obtained were then identified using 1H-NMR, 13C-NMR, distortionless enhancement by polarization transfer, heteronuclear single quantum coherence, and heteronuclear multiple bond coherence. The Activity assay of the isolate was performed using the microdilution method. A pure compound obtained the result of the separation process with eluent n-hexane: Ethyl acetate (3:2) with R_f 0.6. This spot follows the spot in the contact bioautographic result of fraction D, the spot with R_f 0.6 gives an inhibition zone. After identifying and purifying the isolate were known as Robustaflavone, this compound has activity against B. subtilis with a (minimum inhibitory concentration) value of 2500 ppm. Robustaflavone successfully isolated and identified from G. latissima leave and its antibacterial activity.

[FULL TEXT] [PDF]*

Table of Contents

Similar in PUBMED

Search Pubmed for

Search in Google Scholar for

Related articles

Citation Manager

Access Statistics

Reader Comments

Email Alert *

Add to My List *

* Requires registration (Free)

Article Access Statistics

Viewed	738

Printed	24

Emailed	0

PDF Downloaded	74

Comments	[Add]