| ORIGINAL ARTICLE |
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| Year : 2021 | Volume
: 12
| Issue : 4 | Page : 373-377 |
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Characterization and cytotoxicity of low-molecular-weight chitosan and chito-oligosaccharides derived from tilapia fish scales
Gul-e-Saba Chaudhry1, CS Thirukanthan1, K Murni NurIslamiah1, YY Sung1, T S. M. Sifzizul1, A W. M. Effendy2
1 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala, Terengganu, Malaysia
2 Institute of Marine Biotechnology, Universiti Malaysia Terengganu; Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala, Terengganu, Malaysia
Correspondence Address:
A W. M. Effendy
Faculty of Fisheries and Food Science, University Malaysia Terengganu, 21030 Kuala, Terengganu
Malaysia
Dr. Gul-e-Saba Chaudhry
Institute of Marine Biotechnology, University Malaysia Terengganu, 21030 Kuala, Terengganu
Malaysia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/japtr.japtr_117_21
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The present study evaluated the physicochemical characterization and cytotoxicity activity of chitosan and chito-oligosaccharides (COSs). The extraction of chitosan and COSs was executed by chemical hydrolysis. The physicochemical characterization and deacetylation (DA) value were determined using an FTIR. The molecular weight was determined by using the Mark–Houwink equation. The physical parameters such as solubility, water-binding capacity (WBC), and fat-binding capacity (FBC) were determination as per equation (i), (ii), and (iii) respectively. The cytotoxic activities of chitosan and COS against MCF-7, HepG2, HeLa-6, and 3T3 were performed by MTS assay. The COS induced enhance cytotoxicity with IC50 0.87 and 2.21 mg/ml against MCF-7 and HepG2 respectively. However, COSs seem to be more sensitive toward the cell lines with the relative potential of MCF-7 > HepG2 > HeLa. Hence, the results showed promising future perspectives of chitosan and COS to develop biodegradable, antibacterial, cytotoxic naturally derived polysaccharides for cancer drug delivery and smart wound dressings.
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