|Year : 2021 | Volume
| Issue : 4 | Page : 340-344
Prospects for using Origanum Syriacum (L.) as a source of antimicrobial agents
Ranim Mohamad, Ramadan Mussa, Svetlana N Suslina
Department of General Pharmaceutical and Biomedical Technology, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia
|Date of Submission||22-Apr-2021|
|Date of Decision||17-Jun-2021|
|Date of Acceptance||19-Jul-2021|
|Date of Web Publication||20-Oct-2021|
Mrs. Ranim Mohamad
Department of General Pharmaceutical and Biomedical Technology, Institute of Medicine, Peoples' Friendship University of Russia (RUDN University), 6, Miklukho-Maklaya Street, Moscow 117198, Russian Federation
Source of Support: None, Conflict of Interest: None
Origanum syriacum (O. syriacum) (L.) belongs to Group B of the genus Origanum from (Lamiaceae), which is rich in essential oils that exhibit antimicrobial efficacy, anti-inflammatory efficacy, antioxidant activity, and antitumor efficacy. These processing activities are because of its richness in carvacrol and Thymol. In this article, we will focus on O. syriacum, discussing the antimicrobial efficacy for its essential oil and extracts, in addition, cast light on mechanism of essential oil antimicrobial action. This study was conducted from March 2019 to February 2021. We have analyzed the results of studies on antimicrobial efficacy of a plant O. syriacum in the PubMed, Google Scholar, Elsevier over the past 15 years using keywords. O. syriacum essential oil and its extracts have an extensive antimicrobial efficacy give it a great importance in pharmaceutical and medical purposes.
Keywords: Antimicrobial effectiveness, carvacrol, essential oils, Origanum Syriacum (L.), thymol
|How to cite this article:|
Mohamad R, Mussa R, Suslina SN. Prospects for using Origanum Syriacum (L.) as a source of antimicrobial agents. J Adv Pharm Technol Res 2021;12:340-4
|How to cite this URL:|
Mohamad R, Mussa R, Suslina SN. Prospects for using Origanum Syriacum (L.) as a source of antimicrobial agents. J Adv Pharm Technol Res [serial online] 2021 [cited 2021 Dec 5];12:340-4. Available from: https://www.japtr.org/text.asp?2021/12/4/340/328623
| Introduction|| |
Antibiotic resistance is one of the most difficult problems facing the world today; there is currently a shortage of effective antibiotics, which requires the development of new antibiotics and to search for alternative solutions., The efficacy of thousands of plants against bacteria has known since ancient times. However, few extracts have been studied on humans and animals to ensure their safety and effectiveness. This study sheds light on Origanum syriacum (Lamiaceae) plant belongs to Group B of the genus Origanum grows in the Mediterranean region.,, Estimated plant height 30–90 cm, leaves ovate, acute or obtuse, the veins are visible at the bottom surface of the leaf, the leaves are covered with glandular popper, Corolla white, the plant blooms in spring. O. syriacum is used in alternative medicine for processing respiratory diseases: a cough remedy, antispasmodic, carminative, painkiller, anthelmintic, treat respiratory infections, and other bacterial, fungal, and viral diseases., Recent studies have also proven its antioxidant effect, anticancer, antibacterial, antifungal, and anti-acetylcholine oxidant.,,,,,,,,, Efficacy against colon cancer and breast cancer was also confirmed. These medicinal properties are because of its richness in carvacrol (it can be up to79.8% from the content of essential oils) and Thymol (it can be up to 83, 8%). Moreover, 11 flavonoids were detected in the plant. Many studies recently have confirmed its antibacterial effectiveness. We found that most of the studies were done on essential oil, and very few were on alcoholic extracts, we will present the types of bacteria fungicide and viruses that O. syriacum has shown to be effective against them, with mention of the plant's parts used in these studies and focus on the mechanism of essential oil antimicrobial action.
| Materials and Methods|| |
We have analyzed the results of microbial laboratory studies on antimicrobial efficacy of a plant O. syriacum in the PubMed database, Google Scholar and Elsevier over the past 10 years using keywords (O. syriacum (L.), essential oils, O. syriacum antimicrobial activity, Origanum, Origanum antibacterial activity) and also studied the literature data for the last 15 on the uses of the plant in folk medicine. This study was conducted from March 2019 to February 2021.
| Results|| |
The antimicrobial efficacy of the essential oils is caused by phenolic and monoterpene components, the most important of them are carvacrol and thymol and carvacrol has been reported to be one of the most effective antimicrobials., The antimicrobial efficacy of these oils is because of their hydrophobic structure and chemical composition, as hydrophobic properties facilitate the penetration of these substances into the bacterial cell membrane, which leads to a change in the arrangement of lipids and the cytoplasmic membrane, it leads to a change in the cell membrane, which, in turn, leads to alter in the chemical and physical properties of the cell membrane, an increasing of proton passive flux across the cell membrane, electron flow in the cell, active transport, and coagulation in the cell contents.,, Importance of hydroxyl group and its effective role in the antimicrobial activity return to these two compounds was also proved. A delocalized electron system allowing hydroxyl group to release proton. Moreover, many studies confirmed the synergistic effect between essential oil components., O. syriacum effects on many kinds of Gram-positive and Gram-negative bacteria, most of the studies were done on essential oil and very few studies used alcoholic extracts., [Table 1] shows the types of bacteria that O. syriacum proved to be effective on them, the plant part that was used in the study, the extract or the essential oil.
|Table 1: Antibacterial activity, origanum syriacum, with activity against many types of bacteria|
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O. syriacum essential oil is used in traditional medicine to treat skin fungus. Its essential oil and ethanol extracts showed efficacy against a large number of fungi,,, [Table 2]. Several studies confirmed that the cytotoxic nature of essential oils is due to their lipophilic nature that enables them to penetrate the cell wall and cell membrane. Membranes damage mitochondria ceased the formation of acetyl-CoA, which leads to inhibition of aflatoxin biosynthesis and coagulate the cytoplasm, thus damage to the cellular organelles and they also strongly negatively affect the membrane by interacting with cellular ions or active sites of enzymes that depleting the ATP pool., Effect mechanism that essential oil works on fungal strains appears to be similar to the way it works against bacteria where the treatment of A. flavus fungus with Mentha entate essential oil led to leakage calcium, magnesium, and potassium ions, the occurrence of damaged fungal cell structure and reduced ergosterol content of the cell membrane has a strong role in maintaining the durability of the fungal cell membrane.
|Table 2: The essential oils and ethanol extracts from origanum syriacum showed efficacy against many types of fungi|
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O. syriacum has been used against colds and influenza since ancient times. However, evidence and studies proving its anti-viral effectiveness are still scarce. O. syriacum essential oils showed anti-herpes effectiveness against Type I. In other studies, ethanol extracts of O. syriacum showed anti-HSV-1.
| Discussion|| |
Many studies have confirmed that most antibiotics, especially aminoglycosides, have efficacy against many bacteria in vitro, and they are not effective against the intracellular pathogens, Activity of thymol on Salmonella typhimurium has explained in vivo because thymol action fights bacteria is through crossing the cell membrane.,, Thymol is able to protect macrophages from death due to oxidative stress. Thymol and carvacrol act in various roles against many bacteria species, thymol more toxic against Staphylococcus aureus than carvacol, but carvacrol is the most toxic against Escherichia coli. It is important to remember that there are two types of the plant: one contains mainly carvacrol whereas the other contains mainly thymol. Studies have confirmed the importance of harvest time, type of soil, and the effect of climate on the quantity and quality of the essential oil,
| Conclusion|| |
We can suggest that O. syriacum essential oil and its extracts have an extensive antimicrobial efficacy give it a great importance in pharmaceutical and medical purposes.
This article has been supported by the RUDN University Strategic Academic Leadership Program.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Frieri M, Kumar K, Boutin A. Antibiotic resistance. J Infect Public Health 2017;10:369-78.
Church NA, McKillip JL. Antibiotic resistance crisis: Challenges and imperatives. Biologia 2021;19:1-6.
Sen B. Potentiality and possibility of Medicinal Plants on Ayurvedic Principle in prevention and treatment of COVID-19. J Ayurvedic Herb Med 2020;6:100-7.
Kimera F, Sewilam H, Fouad WM, Suloma A. Sustainable production of Origanum syriacum L.
using fish effluents improved plant growth, yield, and essential oil composition. Heliyon 2021;7:e06423.
Al Hafi M, El Beyrouthy M, Ouaini N, Stien D, Rutledge D, Chaillou S. Chemical composition and antimicrobial activity of Origanum libanoticum, Origanum ehrenbergii
, and Origanum syriacum
growing wild in Lebanon. Chem Biodivers 2016;13:555-60.
Kamel MS, Assaf MH, Hasanean HA, Ohtani K, Kasai R, Yamasaki K. Monoterpene glucosides from Origanum syriacum
. Phytochemistry 2001;58:1149-52.
Sadıkoğlu N, Özhatay N. Morphological characteristics of exported taxa as Oregano from Turkey I: Origanum. Istanbul universites Eczacılık Fakultesi Dergisi 2015;45:126-87.
Gutiérrez-Grijalva EP, Picos-Salas MA, Leyva-López N, Criollo-Mendoza MS, Vazquez-Olivo G, Heredia JB. Flavonoids and phenolic acids from oregano: Occurrence, biological activity and health benefits. Plants (Basel) 2017;7:2-24.
Ayman AM, Odeh A, Alobeid B, Boukai H. In vitro
antibacterial activity of Origanum syriacum
L. essential oils against gram-negative bacteria. Avicenna J Clin Microbiol Infect 2019;6:26-30.
Qneibi M, Jaradat N, Hawash M, Zaid AN, Natsheh AR, Yousef R, et al
. The neuroprotective role of Origanum syriacum
L. and Lavandula dentata
L. essential oils through their effects on AMPA receptors. Biomed Res Int 2019;2019:1-11.
Aldisi SS, Jaganjac M, Eid AH, Goktepe I. Evaluation of apoptotic, antiproliferative, and antimigratory activity of Origanum syriacum
against metastatic colon cancer cells. J Herbs Spices Med Plants 2019;25:202-17.
El Gendy AN, Leonardi M, Mugnaini L, Bertelloni F, Ebani VV, Nardoni S, et al.
Chemical composition and antimicrobial activity of essential oil of wild and cultivated Origanum syriacum
plants grown in Sinai, Egypt. Ind Crops Prod 2015;67:201-7.
Spyridopoulou K, Fitsiou E, Bouloukosta E, Tiptiri-Kourpeti A, Vamvakias M, Oreopoulou A, et al.
Extraction, chemical composition, and anticancer potential of Origanum onites
L. essential oil. Molecules 2019;24:2612-27.
Lakis Z, Mihele D, Nicorescu I, Vulturescu V, Udeanu DI. The antimicrobial activity of Thymus vulgaris
and Origanum syriacum
essential oils on Staphylococcus aureus, Streptococcus pneumoniae
and Candida albicans
. Farmacia 2012;60:857-65.
Benelli G, Pavela R, Petrelli R, Cappellacci L, Bartolucci F, Canale A, et al
. Orig anum syriacum
subsp. syriacum: From an ingredient of Lebanese 'manoushe' to a source of effective and eco-friendly botanical insecticides. Ind Crops Prod 2019;134:26-32.
Hassan YA, Khedr AI, Alkabli J, Elshaarawy RF, Nasr AM. Co-delivery of imidazolium Zn (II) salen and Origanum syriacum
essential oil by shrimp chitosan nanoparticles for antimicrobial applications. Carbohydr Polym 2021;260:117834.
Shamseddine L, Chidiac JJ. Composition's effect of Origanum syriacum
essential oils in the antimicrobial activities for the treatment of denture stomatitis. Odontology 2021;109:327-35.
Alonazi MA, Jemel I, Moubayed N, Alwhibi M, El-Sayed NNE, Bacha AB. Evaluation of the in vitro
anti-inflammatory and cytotoxic potential of ethanolic and aqueous extracts of Origanum syriacum
and Salvia lanigera
leaves. Environ Sci Pollut Res Int 2021;28:19890-900.
Ayesh BM, Abed AA, Faris DM. In vitro
inhibition of human leukemia THP-1 cells by Origanum syriacum
L. and Thymus vulgaris
L. extracts. BMC Res Notes 2014;7:612.
Zgheib R, Chaillou S, Ouaini N, Kassouf A, Rutledge D, El Azzi D, et al.
Chemometric tools to highlight the variability of the chemical composition and yield of Lebanese Origanum syriacum
L. essential oil. Chem Biodivers 2016;13:1326-47.
El-Desouky SK, Ibrahim LF, Kawashty SA, El-Ansari MA, Kim YS, Chong HS, et al
. Phytochemical constituents and biological activities of Origanum syriacum
. Z Naturforsch B 2009;64:447-51.
Ben Arfa A, Combes S, Preziosi-Belloy L, Gontard N, Chalier P. Antimicrobial activity of carvacrol related to its chemical structure. Lett Appl Microbiol 2006;43:149-54.
Cristani M, D'Arrigo M, Mandalari G, Castelli F, Sarpietro MG, Micieli D, et al.
Interaction of four monoterpenes contained in essential oils with model membranes: Implications for their antibacterial activity. J Agric Food Chem 2007;55:6300-8.
Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W. Essential oils' chemical characterization and investigation of some biological activities: A critical review. Medicines (Basel) 2016;3:25-41.
Di Pasqua R, Betts G, Hoskins N, Edwards M, Ercolini D, Mauriello G. Membrane toxicity of antimicrobial compounds from essential oils. J Agric Food Chem 2007;55:4863-70.
Chauhan AK, Kang SC. Thymol disrupts the membrane integrity of Salmonella
ser. typhimurium in vitro
and recovers infected macrophages from oxidative stress in an ex vivo
model. Res Microbiol 2014;165:559-65.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils – A review. Food Chem Toxicol 2008;46:446-75.
Alma MH, Mavi A, Yildirim A, Digrak M, Hirata T. Screening chemical composition and in vitro
antioxidant and antimicrobial activities of the essential oils from Origanum syriacum
L. growing in Turkey. Biol Pharm Bull 2003;26:1725-9.
Viuda-Martos M, El Gendy Ael-N, Sendra E, Fernández-López J, Abd El Razik KA, Omer EA, et al.
Chemical composition and antioxidant and anti-Listeria activities of essential oils obtained from some Egyptian plants. J Agric Food Chem 2010;58:9063-70.
Tepe B, Daferera D, Sökmen M, Polissiou M, Sökmen A. The in vitro
antioxidant and antimicrobial activities of the essential oil and various extracts of Origanum syriacum
L var bevanii. J Sci Food Agric 2004;84:1389-96.
Husein AI, Ali-Shtayeh MS, Jamous RM, Zaitoun SY, Jondi WJ, Zatar NA. Antimicrobial activities of six plants used in Traditional Arabic Palestinian Herbal Medicine. Afr J Microbiol Res 2014;8:3501-7.
Darwish RM, Aburjai TA. Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli.
BMC Complement Altern Med 2010;10:1-8.
Al-Mariri A, Safi M. The antibacterial activity of selected Labiatae (Lamiaceae
) essential oils against Brucella melitensis
. Iran J Med Sci 2013;38:44-50.
Al-Mariri A, Safi M. In vitro
antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iran J Med Sci 2014;39:36-43.
Shehadeh M, Jaradat N, Al-Masri M, Zaid AN, Hussein F, Khasati A, et al.
Rapid, cost-effective and organic solvent-free production of biologically active essential oil from Mediterranean wild Origanum syriacum
. Saudi Pharm J 2019;27:612-8.
Daouk RK, Dagher SM, Sattout EJ. Antifungal activity of the essential oil of Origanum syriacum
L. J Food Prot 1995;58:1147-9.
Zain ME, Awaad AS, Al-Outhman MR, El-Meligy RM. Antimicrobial activities of Saudi Arabian desert plants. Phytopharmacology 2012;2:106-13.
Basak S, Guha P. A review on antifungal activity and mode of action of essential oils and their delivery as nano-sized oil droplets in food system. J Food Sci Technol 2018;55:4701-10.
Dwivedy AK, Kumar M, Upadhyay N, Prakash B, Dubey NK. Plant essential oils against food borne fungi and mycotoxins. Curr Opin Food Sci 2016;11:16-21.
Dwivedy AK, Prakash B, Chanotiya CS, Bisht D, Dubey NK. Chemically characterized Mentha cardiaca L. essential oil as plant based preservative in view of efficacy against biodeteriorating fungi of dry fruits, aflatoxin secretion, lipid peroxidation and safety profile assessment. Food Chem Toxicol 2017;106:175-84.
Vimalanathan S, Hudson J. Anti-influenza virus activities of commercial oregano oils and their carriers. J Appl Pharm Sci 2012;2:214-8.
Lai WL, Chuang HS, Lee MH, Wei CL, Lin CF, Tsai YC. Inhibition of herpes simplex virus type 1 by thymol-related monoterpenoids. Planta Med 2012;78:1636-8.
Matza-Porges S, Eisen K, Ibrahim H, Haberman A, Fridlender B, Joseph G. A new antiviral screening method that simultaneously detects viral replication, cell viability, and cell toxicity. J Virol Methods 2014;208:138-43.
Lukas B, Schmiderer C, Franz C, Novak J. Composition of essential oil compounds from different Syrian populations of Origanum syriacum
). J Agric Food Chem 2009;57:1362-5.
Hamed ES. Effect of nitrogenous fertilization and spraying with nano-fertilizer on Origanum syriacum
L. var. syriacum plants under North Sinai conditions. J Pharmacogn Phytochem 2018;7:2902-7.
El-Alam I, Zgheib R, Iriti M, El Beyrouthy M, Hattouny P, Verdin A, et al. Origanum syriacum
essential oil chemical polymorphism according to soil type. Foods 2019;8:90-101.
[Table 1], [Table 2]