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Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F pruski leaves
Vivi Mardina1, Syafruddin Ilyas2, Halimatussakdiah Halimatussakdiah3, Tisna Harmawan3, Masitta Tanjung2, Faridah Yusof4
1 Department of Biology, Faculty of Engineering, Universitas Samudra, 24416 Kota Langsa, Aceh, Indonesia
2 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, 20155, Medan, Indonesia
3 Department of Chemistry, Faculty of Engineering, Universitas Samudra, 24416 Kota Langsa, Aceh, Indonesia
4 Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia
Correspondence Address:
Mrs. Vivi Mardina
Department of Biology, Faculty of Engineering, Universitas Samudra, 24416 Langsa, Aceh
Indonesia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/japtr.JAPTR_131_21
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This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial – using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity – using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 μg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 μg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.
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