|Year : 2021 | Volume
| Issue : 1 | Page : 84-88
The effect of coating chitosan on Porphyromonas gingivalis biofilm formation in the surface of orthodontic mini-implant
Haru Setyo Anggani1, Renaldo Guruh Perdana1, Erwin Siregar1, Endang Winiati Bachtiar2
1 Department of Orthodontic, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
2 Department of Oral Biology, Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
|Date of Submission||14-Jul-2020|
|Date of Decision||03-Sep-2020|
|Date of Acceptance||03-Oct-2020|
|Date of Web Publication||09-Jan-2021|
Dr. Haru Setyo Anggani
Faculty of Dentistry, Universitas Indonesia, Jakarta
Source of Support: None, Conflict of Interest: None
Infection is the main problem for the failure of orthodontic mini-implant. Modern prevention of infection is now focused on local antibacterial coatings on implant devices. Chitosan is biocompatible and has antibacterial properties. Azithromycin is a synthetic antibiotic with immunomodulatory properties in which it has an advantage over the rest of antibiotics. This study aimed to evaluate the effect coating chitosan on the orthodontic mini-implant in Porphyromonas gingivalis biofilm formation. This is an experimental study using 25 orthodontic mini-implants. Five samples were coated with chitosan, 5 samples were coated with chitosan–azithromycin, 5 samples were coated with azithromycin, 5 samples were uncoated, and 5 samples were uncoated and were not exposed to P. gingivalis. P. gingivalis biofilms on the surface of the orthodontic mini-implant were observed after 24 h of incubation. P. gingivalis biofilm mass inhibition was highest in the azithromycin-treated group, followed by chitosan + azithromycin and chitosan only. The one-way ANOVA statistic test and post hoc Bonferroni statistic test of P. gingivalis biofilm mass show a significant difference between and within groups of experiments (P < 0.05). The Pearson correlation test with a value of R = +0.88, indicated that the bacterial viability count and the biofilm mass have a strong positive correlation. In conclusion, orthodontic mini-implant coated with chitosan, chitosan with azithromycin, or azithromycin only effectively suppressed P. gingivalis biofilm formation.
Keywords: Azithromycin, biofilm, chitosan, coating of orthodontic mini-implant, Porphyromonas gingivalis
|How to cite this article:|
Anggani HS, Perdana RG, Siregar E, Bachtiar EW. The effect of coating chitosan on Porphyromonas gingivalis biofilm formation in the surface of orthodontic mini-implant. J Adv Pharm Technol Res 2021;12:84-8
|How to cite this URL:|
Anggani HS, Perdana RG, Siregar E, Bachtiar EW. The effect of coating chitosan on Porphyromonas gingivalis biofilm formation in the surface of orthodontic mini-implant. J Adv Pharm Technol Res [serial online] 2021 [cited 2022 Aug 8];12:84-8. Available from: https://www.japtr.org/text.asp?2021/12/1/84/306569
| Introduction|| |
Orthodontic mini-implants are widely used in the last decade. Several studies have revealed that the orthodontic mini-implant was stable enough against orthodontic forces of 50–250 g. Several other studies also stated that after insertion, orthodontic mini-implants experienced mobility and then dislodged even before administering orthodontic forces. A failure rate of 6.6%–16.1% from orthodontic mini-implants is a greater failure rate than dental implants or other anchoring devices such as miniplate (2.6%–7.3%). Mechanisms that cause the orthodontic mini-implants to fail are still unclear. Several factors considered to play some roles in the failure of orthodontic mini-implant are age, smoking habits, oral hygiene, cortical bone thickness, insertion angle, direction and magnitude of a force, and bacterial contamination.
A previous study stated that the main factor causing implant overdenture's failure is the colonization of the implant surface by pathogenic bacteria. Inflammation of the tissue around the orthodontic mini-implant is believed to be associated with an increase of 30% in orthodontic mini-implant failure.
Bacteria adapt better to the unfavorable external conditions due to the antimicrobial's inability to penetrate the multilayered biofilm matrix and the increase of the antibacterial resistant gene expression.
Porphyromonas gingivalis is the most dominant late colony in peri-implantitis and periodontitis. Peri-implantitis is a progressive and irreversible inflammation in the tissue surrounding the implant body accompanied by bone loss, decreased osseointegration, and is one of the main risk factors for implant overdentures' failure.
One management for peri-implantitis is administering antibiotics either systemically or locally, which must be done wisely and rationally considering bacterial resistance's rapid development. The latest approach to counter bacterial resistance is to use a natural antibacterial originating from nature. One plausible approach considered to be the most effective way to prevent bacterial growth in the implant's tissues is to cover the implant surface with a natural antibacterial coating that also bears biodegradable properties. The antibacterial coating is expected to prevent bacterial attachment on the implant surface, thus preventing biofilm formation.
Azithromycin is effective against Gram-negative bacteria, can penetrate biofilms, and is also believed to be very effective against P. gingivalis strain., Another beneficial feature of azithromycin is its ability to increase body immunity, which is why this antibiotic received considerable attention in the last decade.
Chitosan biopolymer is a natural product that currently has received considerable attention from researchers due to its biocompatibility, bioadhesive, bacteriostatic, and osteoconductive properties., These properties make chitosan now widely researched in the medical world as a wound-healing agent, drug delivery agent, food preservatives, and also as a coating agent for orthopedic and dental implants.
The efficacy of chitosan antibacterial coating on titanium implants has been widely published in orthopedic and denture implant researches., Here, we evaluate the effect of chitosan and azithromycin's orthodontic mini-implants on P. gingivalis biofilm.
| Materials and Methods|| |
This study was an in vitro experimental study. The samples' size was calculated using Federer's sample size estimation formula for unpaired numerical data with normal data distribution. Using Federer's formula, we obtain the number of samples of n >4.75, rounded to 5 samples. Thus, this study will use 5 samples for each treatment group, i.e., coated with chitosan (Sigma-Aldrich, Germany), chitosan + azithromycin (Pfizer, USA), azithromycin, without any coating, and without any coating or cultured in P. gingivalis bacteria (negative control group).
The orthodontic mini-implants used in this study are the mini-implants that have fulfilled the inclusion criteria. The surface of orthodontic mini-implants is made rough with 80 grit sandpaper. The samples are then rinsed with distilled water and ethanol and then dried. After the samples are dry, the mini-implants are sterilized in an autoclave. The sampling was done by random sampling technique.
P. gingivalis was cultured in a Trypticase soy broth media. Bacterial suspension concentration of 106 CFU/ml was prepared in 5 ml per tube for 4 treatment sample groups (1 group – a negative control which was not exposed to bacteria).
Chitosan solutions were prepared at a concentration of 2%–1% acetic acid. Each sample will use 4 ml of this solution. The mixture of chitosan and azithromycin solutions containing 20 ml of 0.5 μg of azithromycin was prepared. All of the solutions used for this study were sterilized using a syringe filter 22 μm.
The biofilm formation in the surface orthodontic mini-implants was rinsed with phosphate-buffered saline two times in order to remove nonsticking bacteria, they were then dried and dissolved the biofilm in the surface of the mini-implant by 96% ethanol solution, and finally, the ethanol elution optical density was measured using ELISA reader (Metertech M965+).
| Results|| |
The results showed that P. gingivalis biofilm mass inhibition was highest in the azithromycin-treated group, followed by chitosan + azithromycin and chitosan only [Figure 1].
|Figure 1: The Porphyromonas gingivalis biofilm mass of the treated group and the negative control|
Click here to view
Post hoc Bonferroni statistic test of P. gingivalis biofilm mass shows that there is a significant difference between and within groups of experiments (P < 0.05) [Table 1].
|Table 1: Post hoc Bonferroni statistic test of Porphyromonas gingivalis biofilm mass|
Click here to view
The viability of P. gingivalis in biofilm was also evaluated, and the result shows that in the azithromycin-treated group, P. gingivalis was comparable to the chitosan + azithromycin-treated group even though in the azithromycin group was the lowest one. The inhibitory effect of the chitosan-only-treated group was lower compared to chitosan + azithromycin and azithromycin [Figure 2].
|Figure 2: The viability Porphyromonas gingivalis in biofilm mass of the treated group and the negative control|
Click here to view
Post hoc Bonferroni statistic test of P. gingivalis viability in biofilms shows that there is a significant difference between and within groups of experiments (P < 0.05) [Table 2].
|Table 2: Post hoc Bonferroni statistic test of Porphyromonas gingivalis viability count (CFU/mL)|
Click here to view
In this study, both the viability of P. gingivalis in biofilm and biofilm mass quantification indicate a similar result, as indicated by the Pearson correlation test with a value of R = + 0.88, which means that the bacterial viability and the biofilm mass have a strong linear positive correlation [Figure 3].
|Figure 3: The correlation between the optical density (610) and colony count (CFU/mL) of Porphyromonas gingivalis in biofilm in the surface of mini-implant orthodontic|
Click here to view
| Discussion|| |
Our study showed that P. gingivalis biofilm mass inhibition was highest in the azithromycin-treated group, followed by chitosan + azithromycin chitosan only. This result is quite obvious, considering that azithromycin is very effective against P. gingivalis bacteria. Besides, the absence of a drug delivery system will cause the antibiotics to release the substrate as quickly as possible to the infected tissue area. Antibiotics without a drug delivery system will release the antibiotic substrate by 65% at the first 1 min and 95% at 5 min; this pattern is advantageous in laboratory studies, however, clinically, it is not necessarily beneficial, because antibiotics cannot fight bacteria for a long time, whereas the incidence of peri-implantitis can occur several days or even weeks after implant placement.
A previous study reported that the antibacterial effectiveness of chitosan depends on several factors, i.e., microbial factors such as the species and numbers, chitosan's intrinsic factors (molecular weight, concentration, and deacetylation level), physical factors such as chitosan solubility in the water, and environmental factors, i.e., acidity (pH), temperature, and reaction time. Thus, it can be safely considered that chitosan biomaterials used in this study have met the ideal criteria. There are pros and cons regarding the antibacterial effectiveness of chitosan against Gram-negative bacteria; Raafat et al., in their study, suggested that chitosan is less effective against Gram-negative bacteria; meanwhile, Ikinci et al. stated that chitosan is effective against anaerobic Gram-negative bacteria P. gingivalis. The results of this study are in line with the results of Ikinci et al., i.e., chitosan can inhibit the growth of P. gingivalis. However, the antibacterial effectiveness of the chitosan group in this study was lower than the azithromycin group and the combination of chitosan–azithromycin. This phenomenon may happen because the antibacterial properties of chitosan are bacteriostatic by destabilizing the cell membrane without killing bacteria.,, Thus, the number of P. gingivalis colonies formed on the implant surface does not necessarily decrease because what happens is only a decrease in the bacteria's virulence only.
The group in which the orthodontic mini-implants were coated with the chitosan–azithromycin combination resulted in an antibacterial potency below the azithromycin group. This result is probably due to one of the properties of chitosan that acts as a slow-release drug delivery agent/controlled delivery drug agent, where chitosan will deliver the drug in controlled conditions so that the drug will have a long therapeutic effect. Norowski, in his study, has stated that a combination of antibiotics and chitosan on implant coating will produce a uniform drug delivery rate for 7 days, after which it continues to decline until the 4th week. Another study by Greene et al. suggests that the combination of chitosan and gentamycin produced a uniform drug delivery rate until day 4. Patel in his study also stated that chitosan with a combination of bioactive glass would deliver ampicillin until the 11th week. It can be concluded that the combination of chitosan with antibiotics would slow down the rate of delivery of antibiotics to the intended area. However, this is a desirable result regarding the implant coating with chitosan and antibiotics; thus, antibiotics will have the maximum efficacy and time to act in the tissues and minimize tissue toxicity risk.
The increasing incidence of bacterial resistance and the scarcity of antibiotic inventions have changed the medical world's paradigm against infection. The old paradigm is all about how to kill bacteria. In contrast, the new paradigm now leads to the use of biomaterials that were believed not to cause any bacterial resistance thanks to their function of only weaken the bacterial virulence by inhibiting the toxin production, blocking the bacterial nutrition, and increasing the host immunity. The further preclinical study is needed in order to confirm whether chitosan only can prevent biofilm formation.
| Conclusions|| |
We conclude that coating chitosan or combined with azithromycin is suppress in vitro the biofilm formation P. gingivalis in the surface mini-orthodontic implant.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Reynders R, Ronchi L, Bipat S. Mini-implants in orthodontics: A systematic review of the literature. Am J Orthod Dentofacial Orthop 2009;135:564.e1-19.
Liou EJ, Pai BC, Lin JC. Do miniscrews remain stationary under orthodontic forces? Am J Orthod Dentofacial Orthop 2004;126:42-7.
Luzi C, Verna C, Melsen B. Immediate loading of orthodontic mini-implants: A histomorphometric evaluation of tissue reaction. Eur J Orthod 2009;31:21-9.
Antoszewska J, Papadopoulos MA, Park HS, Ludwig B. Five-year experience with orthodontic miniscrew implants: A retrospective investigation of factors influencing success rates. Am J Orthod Dentofacial Orthop 2009;136:158.e1-10.
Schätzle M, Männchen R, Zwahlen M, Lang NP. Survival and failure rates of orthodontic temporary anchorage devices: A systematic review. Clin Oral Implants Res 2009;20:1351-9.
Kuroda S, Tanaka E. Risks and complications of miniscrew anchorage in clinical orthodontics. Jpn Dent Sci Rev 2014;50:79-85.
Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infect 2009;72:104-10.
Miyawaki S, Koyama I, Inoue M, Mishima K, Sugahara T, Takano-Yamamoto T. Factors associated with the stability of titanium screws placed in the posterior region for orthodontic anchorage. Am J Orthod Dentofacial Orthop 2003;124:373-8.
Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol 2002;292:107-13.
Persson GR, Renvert S. Cluster of bacteria associated with peri-implantitis. Clin Implant Dent Relat Res 2014;16:783-93.
Mahato N, Wu X, Wang L. Management of peri-implantitis: A systematic review, 2010-2015. Springerplus 2016;5:1-9.
Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st
century. Perspect Medicin Chem 2014;6:25-64.
Coates AR, Hu Y. Novel approaches to developing new antibiotics for bacterial infections. Br J Pharmacol 2007;152:1147-54.
Zhang BG, Myers DE, Wallace GG, Brandt M, Choong PF. Bioactive coatings for orthopaedic implants-recent trends in development of implant coatings. Int J Mol Sci 2014;15:11878-921.
O'Rourke VJ. Azithromycin as an adjunct to non-surgical periodontal therapy: A systematic review. Aust Dent J 2017;62:14-22.
Haffajee AD, Torresyap G, Socransky SS. Clinical changes following four different periodontal therapies for the treatment of chronic periodontitis: 1-year results. J Clin Periodontol 2007;34:243-53.
Bartold PM, du Bois AH, Gannon S, Haynes DR, Hirsch RS. Antibacterial and immunomodulatory properties of azithromycin treatment implications for periodontitis. Inflammopharmacology 2013;21:321-38.
Rabea EI, Badawy ME, Stevens CV, Smagghe G, Steurbaut W. Chitosan as antimicrobial agent: Applications and mode of action. Biomacromolecules 2003;4:1457-65.
Ikono R, Li N, Pratama NH, Vibriani A, Yuniarni DR, Luthfansyah M, et al
. Enhanced bone regeneration capability of chitosan sponge coated with TiO2
nanoparticles. Biotechnol Rep (Amst) 2019;24:e00350.
Azuma K, Izumi R, Osaki T, Ifuku S, Morimoto M, Saimoto H, et al
. Chitin, chitosan, and its derivatives for wound healing: Old and new materials. J Funct Biomater 2015;6:104-42.
Ordikhani F, Simchi A. Long-term antibiotic delivery by chitosan-based composite coatings with bone regenerative potential. Appl Surf Sci 2014;317:56-66.
Jianglian D. Application of chitosan based coating in fruit and vegetable preservation: A review. J Food Process Technol 2013;4:5-8.
Bumgardner JD, Wiser R, Gerard PD, Bergin P, Chestnutt B, Marin M, et al
. Chitosan: Potential use as a bioactive coating for orthopaedic and craniofacial/dental implants. J Biomater Sci Polym Ed 2003;14:423-38.
Qin L, Dong H, Mu Z, Zhang Y, Dong G. Preparation and bioactive properties of chitosan and casein phosphopeptides composite coatings for orthopedic implants. Carbohydr Polym 2015;133:236-44.
Romanò CL, Scarponi S, Gallazzi E, Romanò D, Drago L. Antibacterial coating of implants in orthopaedics and trauma: A classification proposal in an evolving panorama. J Orthop Surg Res 2015;10:157.
Maezono H, Noiri Y, Asahi Y, Yamaguchi M, Yamamoto R, Izutani N, et al
. Antibiofilm effects of azithromycin and erythromycin on Porphyromonas gingivalis
. Antimicrob Agents Chemother 2011;55:5887-92.
Gimeno M, Pinczowski P, Pérez M, Giorello A, Martínez Mà, Santamaría J, et al
. A controlled antibiotic release system to prevent orthopedic-implant associated infections: An in vitro
study. Eur J Pharm Biopharm 2015;96:264-71.
Swanson TE, Cheng X, Friedrich C. Development of chitosan-vancomycin antimicrobial coatings on titanium implants. J Biomed Mater Res A 2011;97:167-76.
Greene AH, Bumgardner JD, Yang Y, Moseley J, Haggard WO. Chitosan-coated stainless steel screws for fixation in contaminated fractures. Clin Orthop Relat Res 2008;466:1699-704.
Raafat D, Sahl HG. Chitosan and its antimicrobial potential-A critical literature survey. Microb Biotechnol 2009;2:186-201.
Ikinci G, Senel S, Akincibay H, Kaş S, Erciş S, Wilson CG, et al
. Effect of chitosan on a periodontal pathogen Porphyromonas gingivalis
. Int J Pharm 2002;235:121-7.
Raafat D. Chitosan as an Antimicrobial Compound: Modes of Action and Resistance Mechansisms; 2008. p. 195.
Ikono R, Mardliyati E, Agustin IT, Ulfi MM, Andrianto D, Hasanah U, et al
. Chitosan-PRP nanosphere as a growth factors slow releasing device with superior antibacterial capability. Biomed Phys Eng Express 2018;4:1-7.
Bachtiar EW, Soejoedono RD, Bachtiar BM, Henrietta A, Farhana N, Yuniastuti M. Effects of soybean milk, chitosan, and anti-Streptococcus mutans IgY in malnourished rats' dental biofilm and the IgY persistency in saliva. Interv Med Appl Sci 2015;7:118-23.
Pier GB. Molecular mechanisms of microbial pathogenesis. In: Harrison's Principles of Internal Medicine, 20e. Jameson JL, Fauci AS, Kasper DL, Hauser SL, Longo DL, Loscalzo J. editors. Principal of internal medicine. 2015. New York: McGraw-Hill Chapter-116.
Norowski PA, Courtney HS, Babu J, Haggard WO, Bumgardner JD. Chitosan coatings deliver antimicrobials from titanium implants: A preliminary study. Implant Dent 2011;20:56-67.
Patel KD, El-Fiqi A, Lee HY, Singh RK, Kim DA, Lee HY, et al
. Chitosan–nanobioactive glass electrophoretic coatings with bone regenerative and drug delivering potential. J Mater Chem 2012;22:24956.
Spellberg B, Gilbert DN. The future of antibiotics and resistance: A tribute to a career of leadership by John Bartlett. Clin Infect Dis 2014;59 Suppl 2:S71-5.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2]
|This article has been cited by|
||Mechanism of action, resistance, synergism, and clinical implications of azithromycin
| ||Mohsen Heidary, Ahmad Ebrahimi Samangani, Abolfazl Kargari, Aliakbar Kiani Nejad, Ilya Yashmi, Moloudsadat Motahar, Elahe Taki, Saeed Khoshnood |
| ||Journal of Clinical Laboratory Analysis. 2022; |
|[Pubmed] | [DOI]|
||Antibacterial Activity of Propolis-Embedded Zeolite Nanocomposites for Implant Application
| ||Jun Sik Son,Eun Ju Hwang,Lee Seong Kwon,Yong-Gook Ahn,Byung-Kwon Moon,Jin Kim,Douk Hoon Kim,Su Gwan Kim,Sook-Young Lee |
| ||Materials. 2021; 14(5): 1193 |
|[Pubmed] | [DOI]|