Synergistic effect of ampicillin and dihydrobenzofuran neolignans (myticaganal C) identified from the seeds of Myristica fragrans Houtt. against Escherichia coli

Kanokrat Keawchai; Parinuch Chumkaew; Patima Permpoonpattana; Theera Srisawat

doi:10.4103/japtr.JAPTR_85_20

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ORIGINAL ARTICLE

Year : 2021 | Volume : 12 | Issue : 1 | Page : 79-83

Synergistic effect of ampicillin and dihydrobenzofuran neolignans (myticaganal C) identified from the seeds of Myristica fragrans Houtt. against Escherichia coli

Kanokrat Keawchai¹, Parinuch Chumkaew¹, Patima Permpoonpattana², Theera Srisawat²
¹ Department of Agricultural Science and Technology, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand
² Department of Agricultural Science and Technology, Faculty of Science and Industrial Technology; Department of Agricultural Science, Faculty of Innovative Agriculture and Fisheries, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand

Correspondence Address:
Dr. Theera Srisawat
Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, 31 Moo 6, Tambol Makham Tia, Muang Surat Thani District, Surat Thani 84000; Faculty of Innovative Agriculture and Fisheries, Prince of Songkla University, Surat Thani Campus, 31 Moo 6, Tambol Makham Tia, Muang Surat Thani District, Surat Thani 84000
Thailand

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/japtr.JAPTR_85_20

The present study was designed to enhance the antibacterial activity of ampicillin against Escherichia coli by combining it with myticaganal C. Antibacterial activity of ampicillin combined with myticaganal C against E. coli was assessed by agar well diffusion. Minimum inhibitory concentrations (MICs) and synergy by checkerboard assay of ampicillin and myticaganal C were assessed by resazurin-based 96-well microdilution. Bacterial responses were assessed by flow cytometry. Ampicillin in combination with myticaganal C showed better zone of inhibition (31.67 ± 0.58 mm) than myticaganal C or ampicillin alone. MIC of ampicillin was found to be 12.5 μg/mL, but myticaganal C was ineffective against E. coli. Myticaganal C (8000 μg/mL) with ampicillin (0.0975 μg/mL) exhibited strong synergy, so the need for ampicillin was reduced 128-fold. Combination inhibited E. coli by acting on cell membrane and by granularity disruptions. These findings indicate that myticaganal C enhances the potential of ampicillin against E. coli, thus providing an effective alternative to deal with the problem of bacterial resistance.

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