|Year : 2020 | Volume
| Issue : 4 | Page : 233-237
Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway
Gul-e-Saba Chaudhry1, Nurul Huda Rahman1, Vigneswari Sevakumaran2, Aziz Ahmad2, Habsah Mohamad1, Muhammad Naveed Zafar3, Yeong Yik Sung1, Tengku Sifzizul Tengku Muhammad1
1 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia
2 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia
3 Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan
|Date of Submission||18-Jun-2020|
|Date of Decision||21-Jul-2020|
|Date of Acceptance||05-Sep-2020|
|Date of Web Publication||10-Oct-2020|
Dr. Gul-e-Saba Chaudhry
Institute of Marine Biotechnology, University Malaysia Terengganu, 21030 Kuala Terengganu
Tengku Sifzizul Tengku Muhammad
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030
Source of Support: None, Conflict of Interest: None
Breast cancer is among the frequently occurring cancer worldwide. The foremost underline aim of this study was to determine the growth inhibitory effect along with mechanistic study of a Bruguiera gymnorrhiza extract on MCF-7. The cytotoxicity activity was determined by using the MTS assay. Butanol extract exhibited the maximum cytotoxicity activity against the MCF-7 cells with IC50of 3.39 μg/mL, followed by diethyl ether and methanol extract (IC50at 16.22 μg/mL and 37.15 μg/mL, respectively) at 72 h. The DeadEndTM Colorimetric Apoptosis Detection System confirmed the induction of apoptosis (via DNA fragmentation) in MCF-7 cells. Both butanol and diethyl ether extracts of B. gymnorrhiza significantly increase the caspase-3 level. However, the diethyl ether extract induced higher caspase-9 levels compared to caspase-8, suggesting that the intrinsic pathway was the major route in the process of apoptosis. Thin-layer chromatography profiling demonstrated the presence of phenolic, terpene, and alkaloid compounds in crude methanol, diethyl ether, and butanol extracts. The phytochemicals present in the extracts of B. gymnorrhiza might have the potential to be a future therapeutic agent against breast cancer.
Keywords: Apoptosis, Bruguiera gymnorrhiza, cancer, caspases, DNA fragmentation, human breast cancer, phytochemicals, TUNEL assay
|How to cite this article:|
Chaudhry Ge, Rahman NH, Sevakumaran V, Ahmad A, Mohamad H, Zafar MN, Sung YY, Tengku Muhammad TS. Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway. J Adv Pharm Technol Res 2020;11:233-7
|How to cite this URL:|
Chaudhry Ge, Rahman NH, Sevakumaran V, Ahmad A, Mohamad H, Zafar MN, Sung YY, Tengku Muhammad TS. Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway. J Adv Pharm Technol Res [serial online] 2020 [cited 2022 Dec 7];11:233-7. Available from: https://www.japtr.org/text.asp?2020/11/4/233/297700
Gul-e-Saba Chaudhry and Tengku Sifzizul Tengku Muhammad are contributed equally to this work.
| Introduction|| |
Breast cancer is among the frequently occurring cancer in men and women. In 2018, approximately 15% of all cancer deaths were due to breast cancer among women. The most common treatments available for breast cancers are chemotherapy, radiotherapy, and surgical treatment. However, chemotherapy and radiotherapy treatments yield adverse side effects which can be overcome by using targeted drug delivery systems and potential phytochemical-based drugs.,,
Based on the World Health Organization report, a major percentage of the world's population utilizes plant-derived medicines for health care. Plant-derived medicines or natural drugs, which form the basis of traditional medicine, have been used for centuries by different cultures. The significance of medicines of natural origin has satisfactorily understood in the pharmaceutical industry. Various Mangrove species produce secondary metabolites with various bioactive properties such as insecticidal, antibacterial, antidiarrheal, cytotoxic, and antiviral.,,,,,,,,,
The main purpose of a therapeutic drug in cancer treatment is to kill the cancer cell without harming normal cells. In cell death mechanism, apoptosis gains much importance due to safely triggering the suicidal process in cancer cells without affecting normal cells. Apoptosis is an extremely regulated mechanism that shows a distinct role in the process of cell growth, death, and development. Cells undergoing apoptosis possess morphological and biochemical alterations such as condensation of chromatin material and cytoplasm shrinkage, phosphoserine exposure, and DNA fragmentation.
Therefore, in this study, the cytotoxicity effects and cell death mechanisms of the extracts of mangrove plant Bruguiera gymnorrhiza on MCF-7 were investigated. Bioactive compounds present in B. gymnorrhiza show an essential part in inhibiting the cell growth apoptosis mechanism by the activation of caspases, thus potentially developed as one of the candidates of chemotherapeutic agents with minimal side effects.
| Materials and Methods|| |
Collection of Bruguiera gymnorrhiza and extract preparation
The leaves of B. gymnorrhiza were collected from Umbai, Malacca (N 02° 09' 330” E 102° 20' 99”). The preparation of extracts according to our formerly reported methods was done using methanol, diethyl ether, and butanol solvents.,
The cytotoxicity of extracts was determined by using CellTiter 96 AQueous One Solution Proliferation assay (MTS) as a formerly reported method.,, The MCF-7 cell line was cultured in ISO-certified Animal Cell Culture Laboratory, (MS/ISO/IEC 17025:2015 SAMM No. 796), Institute of Marine Biotechnology. The absorbance was observed at 490 nm using a plate reader (enzyme-linked immunosorbent assay Multiskan, Thermo Fisher, USA).
DNA fragmentation through TUNEL system (late apoptosis)
DNA fragmentation study was performed by using the DeadEnd™ Colorimetric Detection System (Promega, Madison US). The MCF-7 cells were cultured in an 8-well slide chamber and then incubated in 5% (v/v) CO2 incubator at 37°C for 24 h. The experiment was performed according to a previously reported method., The dark stain of fragmented DNA represents the induction of apoptosis, which was observed by an inverted microscope (Olympus, Selangor Malaysia).
The activation level of caspases 3/7, Caspase-8 and Caspase-9 was determined by using Caspase-GloTM Assay (Promega, USA). The assay was performed according to a previously described method., The breast cancer cell line was treated with active partition extracts of B. gymnorrhiza at a concentration of IC50 at 72 h and incubated for several time points (0 h–36 h) at 37°C. The reading of each sample was measured using a luminometer at Optical density (OD) of 490 nm.
Thin-layer chromatography profiling
Thin-layer chromatography (TLC) analysis was done on TLC silica gel plates (60 F254) to identify the presence of phytochemicals in the active extracts of B. gymnorrhiza. The B. gymnorrhiza extract was diluted in a mandatory amount of solvent using a previously reported method., The developed plates were air-dried, heated, and observed under ultraviolet light at both 254 nm and 365 nm as well as anisaldehyde and Dragendorff's derivatized reagents.
GraphPad Prism software (GraphPad Software, Inc San Diego, CA) was used to calculate the growth inhibition (IC50). Significant differences were expressed by using analysis of variance and (Dunnett's) test using SPSS software version 20.0 (IBM SPSS, USA, New York, US).
| Results and Discussion|| |
Cytotoxicity effect of the Bruguiera gymnorrhiza extracts on MCF-7
In this study, the cytotoxicity effects of the B. gymnorrhiza methanol, diethyl ether, and butanol extracts on the MCF-7 cell line were investigated. This study demonstrated that B. gymnorrhiza methanol, diethyl ether, and butanol extracts of B. gymnorrhiza produced time-dependent increased inhibitory effects on MCF-7 cells. Interestingly, the significant inhibitory effect was observed when cells were treated with methanol, diethyl ether, and butanol extracts at concentrations of 12.50 μg/mL and 6.25 μg/mL [Figure 1]a, [Figure 1]b, [Figure 1]c. However, diethyl ether and butanol extracts only showed a significant cytotoxicity effect at concentrations of 3.12 μg/mL and lower concentrations. B. gymnorrhiza butanol extract showed the highest cytotoxicity effects with the IC50 value of 3.39 μg/mL, followed by diethyl ether extract (16.22 μg/mL). However, B. gymnorrhiza methanol extract showed an inhibitory effect above 30 μg/mL (37.15 μg/mL). The diethyl ether and butanol extracts of B. gymnorrhiza produce cytotoxicity similar to previous studies.,, Therefore, diethyl ether and butanol extracts were selected for further mode of cell death investigation.
|Figure 1: Growth inhibition percentage of Bruguiera gymnorrhiza (a) methanol, (b) diethyl-ether, and (c) butanol extracts against MCF-7 cell at 24, 48, and 72 h. Each value represented mean ± standard error of the mean of nine replicates (three independent experiments)|
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The apoptotic effects of Bruguiera gymnorrhiza extracts on MCF-7 cell line
The DNA fragmentation in MCF-7 was observed by the dark stain in the nucleus of the cancer cell. 1% (v/v) dimethyl sulfoxide was considered negative control and DNAase as positive, according to data shown in our previous study. Interestingly, dark brown stained nuclei were observed in cells treated with the diethyl ether and butanol extracts over the 36-h treatment period, as revealed in [Figure 2]a, [Figure 2]b, [Figure 2]c, [Figure 2]d, [Figure 2]e, [Figure 2]f. These results strongly denote that both extracts of B. gymnorrhiza killed MCF-7 cell line through apoptosis. The induction of apoptosis is the foremost active strategy to inhibit cell growth and acts as an important effective therapeutic target in drug development.,,,,
|Figure 2: The presence of apoptotic cells in MCF-7 cell line after the treatment of Bruguiera gymnorrhiza: butanol extract 12 h (a), 24 h (b), 36 h (c); diethyl ether extract 12 h (d), 24 h (e), 36 h (f); the image was observed under a light microscope at ×40|
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The effects of Bruguiera gymnorrhiza extracts on caspase-3, 8, and 9 protein levels in MCF-7 cells
The significant pathways responsible for the activation of apoptosis involve activation in caspase expression and activation. The expression level of caspase-3 [Figure 3]a significantly increased in cells that were treated with diethyl ether extracts at various treatment periods (9, 12, 16, and 20 h) and reached its peak at 24 h (2.75 folds) and with butanol extract at 16, 20, and 24 h, with the highest level at 16 h (3.25 folds). Interestingly, the level of caspase-8 [Figure 3]b increased at 12 h by 2.7 folds, reaching its peak at 24 h by 5.92 folds in diethyl ether extract-treated cells. The significant increase in the protein level of caspase-9 [Figure 3]c was also noticed, with the highest activity produced when the cells were treated at 1 h (10 folds). Apoptosis is chiefly controlled by caspases; aspartate-specific cysteine proteases involve in the process of apoptosis (function as both initiators and executioners)., Caspase activation is triggered by two major signaling routes: (i) the extrinsic-death receptor and the (ii) intrinsic-mitochondrial pathways. The induction of apoptosis by anticancer agent might be possible by extrinsic pathway and intrinsic pathway or by both pathways activation.
|Figure 3: The expression of (a) caspase-3, (b) caspase-8, and (c) caspase-9 in MCF-7 cells treated with Bruguiera gymnorrhiza diethyl ether and butanol extracts|
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The results showed the separation of compounds spotted on the TLC plate in different solvent system ratios [Figure 4]a and b]. The results indicate that the extracts contained conjugated carbon double bonds (C = C) and alkaloid. The presence of brown spots on a yellow background indicates the presence of organic compounds. The dark orange, violet, and gray spots represent alkaloids, phenol, and terpenes, respectively. The presence of potential phytochemicals in Xylocarpus moluccensis (mangrove species) possessing cytotoxic activity similar to previously reported studies.,,
|Figure 4: Thin-layer chromatography profiling of Bruguiera gymnorrhiza: (a) diethyl ether extract, (b) butanol extract under; (1) before sprayed, (2) UV254, (3) UV365, (4) iodine vapor, (5) Dragendorff's reagent, and (6) anisaldehyde reagent|
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In our previous study,, it was reported that Xylocarpus sp. (mangrove) successfully induces the process of apoptosis in HeLa cells. Xylocarpus moluccensis induces apoptosis through activation of the extrinsic pathway in HepG2 cell lines. However, in the present study, the major activation route was intrinsic along with the extrinsic pathway, which suggested that mangrove species have the potential to induce apoptosis with both extrinsic and intrinsic pathways. The difference might be due to a particular mechanism activated in specific cell lines. The phytochemical study further confirms the presence of potential phenolic, terpene, and alkaloid compounds in crude methanol, diethyl ether, and butanol extracts. The active compounds present in B. gymnorrhiza might have the potential to be a future therapeutic agent. Further study of isolation of pure compounds from B. gymnorrhiza will be our next priority to study the underlying clear mechanism.
| Conclusion|| |
One of the significant discoveries in cancer research was investigating the cell death mechanism exerted by anticancer chemotherapy. In this study, the extracts of mangrove plant B. gymnorrhiza showed significant cytotoxicity effects with IC50 values at 72 h – the induction of apoptosis was confirmed by DNA fragmentation with the underlying mechanism, mainly due to intrinsic pathways. TLC profiling showed that the extracts contain alkaloid, phenolic, and terpenoid compounds. Thus, from this study, it is understood that there is enormous potential for mangrove extracts prepared from B. gymnorrhiza to develop as chemotherapeutic agents in cancer treatment.
Financial support and sponsorship
The research work was supported by Strategic Research Grant, Universiti Malaysia Terengganu (Vot No. 55197).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Mitra S, Dash R. Natural products for the management and prevention of breast cancer. Evid Based Complement Alternat Med 2018;2018:8324696.
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2018;68:394-424.
Chaudhry GE, Akim A, Zafar, MN, Abdullah MA, Sung YY, Muhammad TST. Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA. J Adv Pharm Technol Res 2020;11:101-6. [Full text]
Gul-e-Saba, Abdullah MA. Polymeric nanoparticle mediated targeted drug delivery to cancer cells. In: Thangadurai D, Sangeetha J, editors. Biotechnology and Bioinformatics: Advances and Applications for Bioenergy, Bioremediation, and Biopharmaceutical Research. NJ: Apple Academic; 2015. p. 1-34.
Bibi SN, Fawzi MM, Gokhan Z, Rajesh J, Nadeem N, Kannan RR, et al
. Ethnopharmacology, phytochemistry, and global distribution of mangroves – A comprehensive review. Mar Drugs 2019;17:231.
Chen JD, Feng DQ, Yang ZW, Wang ZC, Qiu Y, Lin YM. Antifouling metabolites from the mangrove plant Ceriops tagal
. Molecules 2008;13:212-9.
Simlai A, Roy A. Biological activities and chemical constituents of some mangrove species from Sundarban estuary: An overview. Pharmacogn Rev 2013;7:170-8.
Calderón JS, Céspedes CL, Rosas R, Gómez-Garibay F, Salazar JR, Lina L, et al
. Acetylcholinesterase and insect growth inhibitory activities of Gutierrezia microcephala
on fall armyworm Spodoptera frugiperda
. J Biosci 2001;56:382-94.
Abeysinghe PD, Wanigatunge RP, Pathirana RN. Evaluation of antibacterial activity of different mangrove plants extracts. Ruhuna J Sci 2006;1:104-12.
Rouf R, Uddin SJ, Shilpi JA, Alamgir M. Assessment of antidiarrhoeal activity of the methanol extract of Xylocarpus granatum
bark in mice model. J Ethnopharmacol 2007;109:539-42.
Han L, Huang XS, Sattler I, Fu HZ, Grabley S, Lin WH. Two new constituents from mangrove Bruguiera gymnorrhiza. J Asian Nat Prod Res 2007;9:327-31.
Rahman NH, Vigneswari S, Ahmad A, Mohamad H, Muhammad TS. Cytotoxic effects and evidence of apoptosis from Avicennia alba
extracts on human breast cancer cell line (MCF-7). J Sustain Sci Manag 2017;12:80-8.
Chaudhry GS, Sohimi NK, Mohamad H, Zafar MN, Ahmed A, Sung YY, et al
. Induction of apoptosis by selected Xylocarpus sp
., fractions in the human cervical cancer cell line, HeLa. Int J Res Pharm Sci 2020;11:2332-9.
Sari DP, Basyuni M, Hasibuan PA, Sumardi S, Nuryawan A, Wati R. Cytotoxic and antiproliferative activity of polyisoprenoids in seventeen mangroves species against WiDr colon cancer cells. Asian Pac J Cancer Prev 2018;19:3393-400.
Ancheeva E, Daletos G, Proksch P. Lead compounds from mangrove-associated microorganisms. Mar Drugs 2018;16:319.
Jan R, Chaudhry GE. Understanding apoptosis and apoptotic pathways targeted cancer therapeutics. Adv Pharm Bull 2019;9:205-18.
Gul-e-Saba, Ismail M, Ismail N, Mohammad H, Muhammad TS. Induction of apoptosis by Aaptos sp., fractions in human breast cancer cell line, MCF-7. Int J Res Pharm Sci 2018;9:328-37.
Hudaya T, Gul-e-Saba, Taib M, Ismail N, Mohammad TS. Methanol extracts of four selected marine sponges induce apoptosis in human breast cancer cell line, MCF-7. Int J Res Pharm Sci 2017;8:667-75.
Chaudhry GE, Jan R, Mohamad H, Tengku Muhammad TS. Vitex rotundifolia
fractions induce apoptosis in human breast cancer cell line, MCF-7, via extrinsic and intrinsic pathways. Res Pharm Sci 2019;14:273-85.
Yunus U, Zulfiqar MA, Ajmal M, Bhatti MH, Chaudhry GE, Muhammad TS, et al
. Targeted drug delivery systems: Synthesis andin vitro
bioactivity and apoptosis studies of gemcitabine-carbon dots conjugates. Biomed Mater 2020;[published online ahead of print, 2020 May 22]. [Doi: 10.1088/1748-605X/ab95e1].
Zafar MN, Masood S, Chaudhry GS, Muhammad TS, Dalebrook AF, Nazar MF, et al
. Synthesis, characterization and anti-cancer properties of water-soluble bis(PYE) pro-ligands and derived palladium (ii) complexes. Dalton Trans 2019;48:15408-18.
Chaudhry GE, Jan R, Zafar MN, Mohammad H, Muhammad TS. Vitex rotundifolia
fractions induced apoptosis in human breast cancer T-47D cell line via activation of extrinsic and intrinsic pathway. Asian Pac J Cancer Prev 2019;20:3555-62.
Chaudhry GS, Murni NI, Zafar MN, Habsah M, Yosie A, Noraznawati I, et al
. Induction of apoptosis by Stichopus chloronotus
and Holothuria nobilis
fractions in human cervical cancer cell line, HeLa. Int J Res Pharm Sci 2020;11:1238-47.
Suffness M, Pezzuto JM. Assays related to cancer drug discovery. In: Hostettmann K, editor. Methods in Plant Biochemistry: Assays for Bioactivity. Vol. 6. London: Academic Press; 1990. p. 71-133.
Vijayarathna S, Sasidharan S. Cytotoxicity of methanol extracts of Elaeis guineensis
on MCF-7 and Vero cell lines. Asian Pac J Trop Biomed 2012;2:826-9.
Andriani Y, Chaudhry GE, Oksal E, Pangestika I, Ramli NM, Mohamad H, et al
. Antihypercholesterolemic and antiatherosclerotic potencies of Pandanus tectorius
fruits via increasing scavenger receptor-B1 genes expression and inhibition of 3-hydroxy-3-methylglutaryl coenzyme a reductase activity. J Adv Pharm Technol Res 2020;11:30-5.
] [Full text]
Pistritto G, Trisciuoglio D, Ceci C, Garufi A, D'Orazi G. Apoptosis as anticancer mechanism: Function and dysfunction of its modulators and targeted therapeutic strategies. Aging (Albany NY) 2016;8:603-19.
Steele VE. Current mechanistic approaches to the chemoprevention of cancer. J Biochem Mol Biol 2003;36:78-81.
Mahar J, Saeed A, Chaudhry GS, Irfan M, Channar PA, Faisal M, et al
. Synthesis, characterization and cytotoxic studies of novel 1,2,4-triazole-azomethine conjugates. J Iran Chem Soc 2020;17:943-51. [Doi: 10.1007/s13738-019-01826-9].
Zhang W, Couldwell WT, Song H, Takano T, Lin JH, Nedergaard M. Tamoxifen-induced enhancement of calcium signaling in glioma and MCF-7 breast cancer cells. Cancer Res 2000;60:5395-400.
Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol 2007;35:495-516.
Papaliagkas V, Anogianaki A, Anogianakis G, Ilonidis G. The proteins and the mechanisms of apoptosis: A mini-review of the fundamentals. Hippokratia 2007;11:108-13.
Danial NN, Korsmeyer SJ. Cell death: Critical control points. Cell 2004;116:205-19.
Kristanti H, Tunjung WAS. Detection of alkaloid, flavonoid, and terpenoid compounds in bread (Artocarpus communis
Forst) leaves and pulps. Life Sci., 2015;2:13-26.
Shin SA, Moon SY, Kim WY, Paek SM, Park HH, Lee CS. Structure-based classification and anti-cancer effects of plant metabolites. Int J Mol Sci 2018;19:2651.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
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