High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell

Saeed Azandeh; Darioush Bijan Nejad; Vahid Bayati; Foroug Shakoor; Negar Varaa; Bahman Cheraghian

doi:10.4103/japtr.JAPTR_312_18

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ORIGINAL ARTICLE

Year : 2019 | Volume : 10 | Issue : 1 | Page : 9-15

High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell

Saeed Azandeh¹, Darioush Bijan Nejad¹, Vahid Bayati¹, Foroug Shakoor², Negar Varaa³, Bahman Cheraghian⁴
¹ Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
² Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
³ Department of Anatomical Science, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran
⁴ Department of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Iran<

Correspondence Address:
Dr. Saeed Azandeh
Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61335
Iran

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/japtr.JAPTR_312_18

For transplantation of cell into injured tissues, cells should be transferred to the damaged site through an adequate carrier. Nevertheless, the nutrient-limited and hypoxic condition in the carrier can bring about broad cell death. This study set to assess the impact of alginate concentrations on the differentiation and the proliferation of cells encapsulated in alginate hydrogels. Human Wharton's Jelly-derived Mesenchymal Stem Cells (HWJ-MSCs) were encapsulated in two concentrations of alginate hydrogel. Then, the proliferation and the hepatic differentiation were evaluated with an MTT assay and the enzyme-linked immunosorbent assay software and urea production. The results demonstrated that the proliferation of cell and urea production in 1.5% alginate concentration was higher than in 2.5% alginate concentration in the hydrogels of alginate. We deduce that the optimized alginate hydrogel concentration is necessary for achieving comparable cell activities in three-dimensional culture.

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