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 Table of Contents  
Year : 2017  |  Volume : 8  |  Issue : 2  |  Page : 63-66  

Comparison of amoxicillin and metronidazole effect on three-drug regimen for the treatment of Helicobacter pylori infection in children

1 Department of Pediatrics, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Department of Epidemiology and Biostatistics, Shahrekord University of Medical Sciences, Shahrekord, Iran

Date of Web Publication11-Apr-2017

Correspondence Address:
Abolfazl Khoshdel
Biochemistry Research Center, Shahrekord University of Medical Sciences, Rahmatiyeh, Shahrekord
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/japtr.JAPTR_162_16

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Helicobacter pylori is an important risk factor for chronic gastritis, peptic ulcer, and gastric cancer. Three-drug regimen is the first-line treatment for this infection, but the response rate to treatment varies in different geographical regions. This study was conducted to comparatively determine the effect of amoxicillin and metronidazole on three-drug regimen to treat H. pylori infection in 1–15-year-old children. This clinical trial was conducted on 82 patients aged 1–15 years with convenience sampling referring to the Endoscopy Unit of Hajar Hospital, Shahrekord. Group 1 was administered with clarithromycin, amoxicillin, and omeprazole (CAO), and Group 2 with, clarithromycin, metronidazole, and omeprazole (CMO). One month after completion of the treatment, stool antigen test was used to study the eradication of H. pylori. Data were analyzed using SPSS software by Chi-square test. Three of the 82 patients were excluded from the study because of side effects caused by drugs. Nearly 87.2% of the patients in CAO-treated group and 92.5% in CMO-treated group had response to treatment. There was no significant difference in eradication rate between the two regimens (P = 0.43). The two regimens displayed no superiority over each other for eradicating H. pylori infection and response rate to treatment in children aged 1–15 years.

Keywords: Amoxicillin, clarithromycin, Helicobacter pylori, metronidazole, omeprazole

How to cite this article:
Kasiri KA, Khoshdel A, Karimi A, Sedehi M, Kasiri N. Comparison of amoxicillin and metronidazole effect on three-drug regimen for the treatment of Helicobacter pylori infection in children. J Adv Pharm Technol Res 2017;8:63-6

How to cite this URL:
Kasiri KA, Khoshdel A, Karimi A, Sedehi M, Kasiri N. Comparison of amoxicillin and metronidazole effect on three-drug regimen for the treatment of Helicobacter pylori infection in children. J Adv Pharm Technol Res [serial online] 2017 [cited 2023 Apr 1];8:63-6. Available from: https://www.japtr.org/text.asp?2017/8/2/63/204339

  Introduction Top

Helicobacter pylori is a spiral, Gram-negative, microaerophilic bacterium with polar flagella, which was first isolated from the end lining tissue of human stomach in 1982[1]H. pylori is a human stomach-specific pathogenic bacterium which has colonized in at least half of the whole population of the world.[2]

Many studies have reported the decrease in the efficiency of this regimen because of increased resistance to clarithromycin and have recommended a new regimen to be introduced. However, this regimen remains to result in a good eradication in the regions with low prevalence of resistance to H. pylori.[3] We decided to compare the eradication rate of two three-drug regimens, clarithromycin, amoxicillin, and omeprazole (CAO) and clarithromycin, metronidazole, and omeprazole (CMO) for H. pylori infection to investigate their efficacy for H. pylori eradication in Chaharmahal and Bakhtiari province, southwest Iran.

  Materials and Methods Top

This randomized clinical trial was conducted on 82 symptomatic patients with the following inclusion criteria: patients having symptoms (dyspepsia, epigastric pain, gastrointestinal bleeding, etc.) for 1 month to 2 years, not responding to outpatient treatment, and being 1–15 years old. The patients were admitted to the educational center of Hajar hospital, Shahrekord, Southwest Iran, for endoscopy. On performing endoscopy, we obtained two specimens from the patients' gastric tissue. A specimen was used for rapid urease test (RUT) and the other one was sent to the laboratory for pathology examination using hematoxylin and eosin that is the only method that can detect other H. pylori-associated lesions (atrophy, intestinal metaplasia, etc.).[4],[5],[6] In general, serologic assays cannot be used on their own in children and adolescents for either diagnosis of H. pylori infection or to monitor the success of therapy because the sensitivity and specificity for the detection of antibodies (IgG or IgA) against H. pylori in children vary widely.[7] After 24 h, the results of RUT were prepared. All the patients with positive RUT result of gastric tissue biopsy specimens after 24 h or positive pathology results were included in the study. The exclusion criteria were patients with previous treatment for H. pylori infection, antibiotic therapy within 4 weeks prior to endoscopy, malignancy or suspected malignancy, gastrectomy, acquisition of severe hepatic disease or any severe diseases within the past 2 years, and having no compliance with the administered regimens. Patients fulfilling the therapy prerequisites were enrolled and informed about the research and related procedures. The patients were fully informed of the research purposes. Written consent was filled by all patients. Then, the patients enrolled were randomly assigned to two groups of 41 each, underwent treatment in one of the Groups of A or B. The patients in Group A were administered with CAO regimen and those in Group B were administered with CMO. The patients administered with CAO received 1–2 mg/kg omeprazole twice a day for 1 month, and simultaneously 50 mg/kg amoxicillin and 15 mg/kg clarithromycin twice a day for 2 weeks.[8] The patients administered with CMO should receive 1 mg/kg omeprazole twice a day divided into two doses for 1 month and simultaneously 20 mg/kg metronidazole and 15 mg/kg clarithromycin twice a day for 2 weeks.

After the patients completed filling out checklists of the demographic data, they were informed of their next referring which was the date of the completion of regimens taking. The patients were told that they should inform the research team if any problems occur to them, particularly side effects or any other problems leading to the discontinuation of regimens taking. If a patient was not able to follow regimen for any reasons such as side effects, withdrawal from the study, and no tolerance, he/she was excluded from the study.

The patients were revisited after completion of regimens and were told to undergo stool antigen test using monoclonal immunochromatography 1 month later for the detection of H. pylori eradication.[9] The patients were informed of the exact date of stool antigen test and were reminded of not using proton pump inhibitor (PPI) and antibiotics till stool antigen test. The test results were recorded in the patients' checklists. The data of checklists were analyzed using SPSS version 22 (Armonk, NY: IBM Corp.) by chi-square test.

  Results Top

Overall, 82 patients were enrolled in this study, and three patients, two in CAO group and one in CMO group, were excluded from the study because of side effects. The two groups were matched by age and there was no significant difference in age between them [Table 1]. The data analysis indicated that there was no significant difference in H. pylori complete eradication between males and females in the two groups [Tables 2 and 3].
Table 1: The rate of eradication and response to Helicobacter pylori in different age groups

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Furthermore, the findings indicated that the number of males and females with full eradication of H. pylori in the CAO- and CMO-treated groups was not significantly different [Table 2 and 3].
Table 2: Eradication rate of Helicobacter pylori infection in both sexes between the two treatment groups

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Table 3: Rate of eradication and response to Helicobacter pylori treatment in two groups

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Overall, the data analysis, irrespective of age and gender, indicated that the response rate was 87.2% for CAO regimen and 92.5% for CMO regimen with no significant difference in the eradication rate between the two regimens. [Table 3] shows the comparison of the two studied regimens.

Regarding side effects, three patients exhibited intolerance to clarithromycin and no side effects were seen due to amoxicillin and metronidazole in this study. No difference was seen in side effects between the two groups.

  Discussion Top

Long-term single use of clarithromycin for respiratory tract diseases has caused increased resistance to it. Resistance to clarithromycin is the most important reason for failure of H. pylori treatmentand decline in its eradication rate.[10] The resistance rate to clarithromycin was obtained at 11.1%, 18.9%, and 29.3% in 2011 in Europe, Asia, and Americas, respectively.[11] Several studies have found the decline in this regimen efficacy due to increased resistance to clarithromycin.[12],[13],[14]

Metronidazole is used to treat anaerobic bacterial diseases, ameba, giardiasis, oral infections, and vaginosis and it has side effects including nausea, dyspepsia, pain, abdominal cramps, constipation, hairy tongue, glossitis, dry mouth, and bad taste,[15],[16] none of which were seen in the patients of the present study.

Antelo et al. studied 120 patients assigned to three groups in Argentina. The eradication rate of the three regimens was reported to be similar, and the regimen administered for the Group 1 was offered as the best regimen because of fewer side effects.[17] Regarding eradication rate, this study is consistent with the present study. However, the side effects were similar to the present study. A study by Treiber et al. demonstrated that the effect of CMO was compared with that of CMO + amoxicillin on H. pylori eradication, and an effective and safe new regimen for H. pylori eradication with no major side effects was reported.[18]

In a study in China, different three-drug regimens were compared for treatment of the wounds due to H. pylori infection within a 1-year follow-up. The eradication rate of amoxicillin-contained and metronidazole-contained three-drug regimens administered for 14 days was 92.3% and 86%, respectively, and 7-day amoxicillin-contained three-drug regimen was offered as the best regimen because of the lowest recurrence rate of the wounds,[19] which is partially consistent with the present study.

However, in a systematic review of 75 articles, the eradication rate of PPI + amoxicillin + clarithromycin and PPI + metronidazole + clarithromycin regimens was 83.5% and 68.6%, respectively. All the three regimens were administered for 14 days,[20] which could be due to the difference in the studied geographical regions and high prevalence of resistance to metronidazole in these regions.

Moreover, a study by Paoluzi et al. in 2006 in Italy on 486 patients to compare 1- and 2-week three-drug regimens concluded that the eradication rate of the 2-week treatment was much higher than the 1-week treatment, and the eradication rate of amoxicillin-contained three-drug regimen was much higher than that of metronidazole-contained one (70% and 52%, respectively),[21] which is lower than the eradication rate obtained for both regimens in the present study. This could be explained by high resistance to clarithromycin and metronidazole in the area of study.

In a systematic review in Iran, H. pylori resistance to metronidazole, clarithromycin, amoxicillin, was 61.6%, 22.4%, and 16.0%, respectively.[22] In Khademi study in Isfahan, Iran, H. pylori resistance to clarithromycin, metronidazole, and amoxicillin was 15.3%, 55.1%, and 6.4%, respectively.[23]

Inconsistent with the present study, a study by Kutluk et al. reported only 50% of eradication rate in children of 2–15 years of age for CAO.[24] A study in China found 66.4% eradication rate for CAO in children,[19] and a study in Turkey obtained the eradication rate of 64.4%.[24] Culture and sensitivity tests were not conducted in this study that can be considered a limitation of this study.

  Conclusion Top

The comparison of two therapeutic regimens, CAO and CMO, indicated no significant difference in complete recovery and H. pylori eradication between the two regimens. The side effects were approximately similar in the two studied groups. Therefore, both regimens could be used to eliminate H. pylori infection and treat the patients with such infection.


The present study was obtained from a research project at and funded by the Research and Technology Deputy of Shahrekord University of Medical Sciences. We thank the staff of Hajar Hospital, Shahrekord, Clinical Research Unit of this hospital, and other people who assisted us in conducting this study.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

  References Top

Hatakeyama M. Helicobacter pylori and gastric carcinogenesis. J Gastroenterol 2009;44:239-48.  Back to cited text no. 1
Delport W, Cunningham M, Olivier B, Preisig O, van der Merwe SW. A population genetics pedigree perspective on the transmission of Helicobacter pylori. Genetics 2006;174:2107-18.  Back to cited text no. 2
Guarner J, Bartlett J, Whistler T, Pierce-Smith D, Owens M, Kreh R, et al. Can pre-neoplastic lesions be detected in gastric biopsies of children with Helicobacter pylori infection? J Pediatr Gastroenterol Nutr 2003;37:309-14.  Back to cited text no. 3
Kato S, Nakajima S, Nishino Y, Ozawa K, Minoura T, Konno M, et al. Association between gastric atrophy and Helicobacter pylori infection in Japanese children: A retrospective multicenter study. Dig Dis Sci 2006;51:99-104.  Back to cited text no. 4
Ricuarte O, Gutierrez O, Cardona H, Kim JG, Graham DY, El-Zimaity HM. Atrophic gastritis in young children and adolescents. J Clin Pathol 2005;58:1189-93.  Back to cited text no. 5
Guarner J, Kalach N, Elitsur Y, Koletzko S. Helicobacter pylori diagnostic tests in children: Review of the literature from 1999 to 2009. Eur J Pediatr 2010;169:15-25.  Back to cited text no. 6
Cherry JD, Harrison GJ, Kaplan SL, Hotez PJ, Steinbach WJ. Feigin and Cherry's Textbook of Pediatric Infectious Diseases. 7th ed. Philadelphia: Elsevier/Saunders; 2014. p. 1996.  Back to cited text no. 7
Kliegman R, Behrman RE, Nelson WE. Nelson Textbook of Pediatrics. 20th ed. Philadelphia, PA: Elsevier/Saunders; 2016. p. 1818.  Back to cited text no. 8
Mégraud F. H. pylori antibiotic resistance: Prevalence, importance, and advances in testing. Gut 2004;53:1374-84.  Back to cited text no. 9
De Francesco V, Giorgio F, Hassan C, Manes G, Vannella L, Panella C, et al. Worldwide H. pylori antibiotic resistance: A systematic review. J Gastrointestin Liver Dis 2010;19:409-14.  Back to cited text no. 10
Paoluzi OA, Visconti E, Andrei F, Tosti C, Lionetti R, Grasso E, et al. Ten and eight-day sequential therapy in comparison to standard triple therapy for eradicating Helicobacter pylori infection: A randomized controlled study on efficacy and tolerability. J Clin Gastroenterol 2010;44:261-6.  Back to cited text no. 11
Kearney DJ, Brousal A. Treatment of Helicobacter pylori infection in clinical practice in the United States: Results from 224 patients. Dig Dis Sci 2000;45:265-71.  Back to cited text no. 12
Malfertheiner P, Bazzoli F, Delchier JC, Celiñski K, Giguère M, Rivière M, et al. Helicobacter pylori eradication with a capsule containing bismuth subcitrate potassium, metronidazole, and tetracycline given with omeprazole versus clarithromycin-based triple therapy: A randomised, open-label, non-inferiority, phase 3 trial. Lancet 2011;377:905-13.  Back to cited text no. 13
Wallace RJ Jr., Brown BA, Griffith DE. Drug intolerance to high-dose clarithromycin among elderly patients. Diagn Microbiol Infect Dis 1993;16:215-21.  Back to cited text no. 14
Peters DH, Clissold SP. Clarithromycin. A review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential. Drugs 1992;44:117-64.  Back to cited text no. 15
Graham DY, Abudayyeh S, El-Zimaity HM, Hoffman J, Reddy R, Opekun AR. Sequential therapy using high-dose esomeprazole-amoxicillin followed by gatifloxacin for Helicobacter pylori infection. Aliment Pharmacol Ther 2006;24:845-50.  Back to cited text no. 16
Antelo P, Almuzara M, Avagnina A, Topor J, Barberis C, Barcia T, et al. Diagnosis and treatment of Helicobacter pylori infection. Its relationship with gastrointestinal ulcer and antimicrobial resistance. Medicina (B Aires) 2001;61(5 Pt 1):545-51.  Back to cited text no. 17
Treiber G, Ammon S, Schneider E, Klotz U. Amoxicillin/metronidazole/omeprazole/clarithromycin: A new, short quadruple therapy for Helicobacter pylori eradication. Helicobacter 1998;3:54-8.  Back to cited text no. 18
Zhou L, Zhang J, Chen M, Hou X, Li Z, Song Z, et al. A comparative study of sequential therapy and standard triple therapy for Helicobacter pylori infection: A randomized multicenter trial. Am J Gastroenterol 2014;109:535-41.  Back to cited text no. 19
Khademi F, Poursina F, Hosseini E, Akbari M, Safaei HG. Helicobacter pylori in Iran: A systematic review on the antibiotic resistance. Iran J Basic Med Sci 2015;18:2-7.  Back to cited text no. 20
Paoluzi P, Iacopini F, Crispino P, Nardi F, Bella A, Rivera M, et al. 2-week triple therapy for Helicobacter pylori infection is better than 1-week in clinical practice: A large prospective single-center randomized study. Helicobacter 2006;11:562-8.  Back to cited text no. 21
Keshavarz Azizi Raftar S, Moniri R, Saffari M, Razavi Zadeh M, Arj A, Mousavi SG, et al. The Helicobacter pylori resistance rate to clarithromycin in Iran. Microb Drug Resist 2015;21:69-73.  Back to cited text no. 22
Khademi F, Faghri J, Poursina F, Esfahani BN, Moghim S, Fazeli H, et al. Resistance pattern of Helicobacter pylori strains to clarithromycin, metronidazole, and amoxicillin in Isfahan, Iran. J Res Med Sci 2013;18:1056-60.  Back to cited text no. 23
Kutluk G, Tutar E, Bayrak A, Volkan B, Akyon Y, Celikel C, et al. Sequential therapy versus standard triple therapy for Helicobacter pylori eradication in children: Any advantage in clarithromycin-resistant strains? Eur J Gastroenterol Hepatol 2014;26:1202-8.  Back to cited text no. 24


  [Table 1], [Table 2], [Table 3]


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